#aflm | Explore Tumblr Posts and Blogs

Visit Blog

Explore Tumblr blogs with no restrictions, modern design and the best experience.

Fun Fact

Average visit duration of Tumblr.com is 10 mins and 25 secs.

viratfc · 10 hours

Text

Oh my goodness Jamie 🤯

#collingwood #magpies #afl #aflm

13 notes · View notes

fazcinatingblog · 24 days

Text

trent's boyfriend

#aflm

3 notes · View notes

buttbiscuit · 2 years

Text

STOP he's so cute 😭😭😭

#afl #caleb serong #aflm #fremantle dockers #flagmantle

2 notes · View notes

b3crew · 4 months

Text

Duck Amuck in Japan: Episode 08 | Mum's the Word | B3crew.com

It's the first Duck Amuck in Japan episode of 2024, and King Baby Duck, AFLM, and JonStar have got quite a lot of ground to cover. An interview opportunity lands on AFLM's lap, while our host talks about meeting comedian Ron Funches. The twins reflect on their winter break, and KBD shares his report on concerts from Soil & "Pimp" Sessions, The50Kaitenz, and the Guitar Wolf/Polysics double-bill! AFLM says "I do!" to the video game Wedding Witch, and KBD shares what he did when his mom (or "Our Mom", as the bros said) came to visit! Plus, it's also a certain host's birthday today...

0 notes

ancient-string · 11 months

Text

We! Are! Walyalup! Say we are Walyalup!

#kalyakoori walyalup #aflm #afl #australia #footy #walyalup

1 note · View note

solomonadedayo · 1 year

Photo

African Future Leadership Magazine Hits Nigerian Newsstands. The management of a monthly publication, the African Future Leadership Magazine (AFLM), has announced the commencement of the sales of the paper. Publisher of AFLM, Mark Isimenmen Idiahi, while announcing the maiden edition in a press statement made available to Daily Independent at the weekend, said: “The African Future Leadership Magazine (AFLM) is out.” The “scintillating edition, which was launched on Friday, October 28, 2022, in Lagos during the 7th Edition of the Future Leadership Conference and Awards event,” according to Idiahi, was birthed on the aegis of the Future Leadership Conferences, adding: “It is a monthly publication of the The Second Chance CARE Foundation.” While using the statement to invite media practitioners to the special unveiling of AFLM and a press conference scheduled to take place at the Silverbird Cafe of the Silverbird Galaria, Victoria Island, Lagos, on Tuesday, November 8, 2022 at 11am prompt, Mark Idiahi said: “This serves as an official invitation to all our online and offline media partners, print and electronic media, advert agencies, friends, colleagues, and the general public to come and be part of our Special Unveiling of the African Future Leadership Magazine event.” Idiahi, who is passionate about getting leadership right on the African continent, said that AFLM’s mission is to promote excellence in leadership and enhance Africa’s leadership capacities in all spheres of human endeavours, among others. We've also officially launched our website. Kindly visit http://www.africanfutureleadership.com to explore our online platform. #AFLM #FutureLeadershipMagazine (at Lagos, Nigeria) https://www.instagram.com/p/CktvhiMjbZN/?igshid=NGJjMDIxMWI=

#aflm #futureleadershipmagazine

0 notes

ratherembarrassing · 7 months

Text

2023: week 37

the margot robbie bingewatch of 2023: whisky tango foxtrot (2016). what a weird movie. what a weird movie! there are the obvious problems, which were likely the reasons i never saw it at the time, and there is the tina fey of it all (open very long parenthetical: even in shows/films for which she is not credited as a writer but is credited as a producer, there is such a very very very specific vibe about 1. female characters in general, and 2. any character she plays specifically, that it's very hard not to conclude she is the source. and at least it's interesting but christ does she have an angle. even in a movie that is 95% not a comedy, in which she plays a reporter in afghanistan in the 2000s, the primary "joke" of almost all of the "comedy" is about how objectively unattractive she is compared to actually hot women. the fact that this movie managed to actually and convincingly make kim and tanya friends and their ultimate beef was about their work is, I CAN ONLY ASSUME BASED ON ALL OF HISTORY, is in spite of and not because of tina fey's involvement. it's so weird and depressing that she has a filmography long and extensive enough that this is plainly apparent!) but also, somewhere in there is an okay movie dying to escape! anyway, babyface margot robbie again playing a character who could, with the tiniest of adjustments, totally be a lesbian. i have come to appreciate that even in terrible movies she will likely be playing the most interesting character in the dramatis, and that's very fun of her and for me.

also, greta gerwig: little women (2019). what a fucking movie! i cannot begin to tell you how loudly i was moaning when friedrich showed up again (remember, i've never read the book and never seen the 1994 movie). i should read the book, but i fear my other minor issues will become major issues, but in any event i get why jo march is an icon.

sportsball: aflm: getting knocked out in the prelim final by gws of all teams: devastating. aflw: PORT WON A GAME!!!!!!!!!! it's only happened once before, so this was a very confusing turn of events, but we played so well! i'm very glad i drove to fucking moorabbin for this rare and beautiful experience.

some good food: the land is inhospitable and so are we, mitski (2023). mitski in her country era!!!!!!!!!!!!!!!!!! the back to back wallop of heaven (is this to most beautiful song i've ever heard? maybe!) and i don't like my mind!!!!!!!!!!!!!!!! the way she continues to play with volume as a second dimension to her sound continues to fascinate and irritate (girl i got enough hearing problems before you start doing this to me) in equal measure. please tour with a full strong contingent!!!!!!!!!!!!!!! 2019 was so long ago 😭 (will i ever stop regretting not buying that ouija board tshirt because they only took cash and something something the atm? 2019 was so long ago.)

things that made me cry: bunny's return to only murders in the building, if only in flashback: devastating. and uma, my love. this show does such a job with its older characters.

AND ALSO THIS: this barbie dancing to nobody by mitski tiktok is what the inside of my head looks like at all times.

#this week in ashleigh

11 notes · View notes

artemisbarnowl · 1 year

Text

The convo around contact sports and head trauma is here! Kill the aflm tbh.

#the story of an enquiry and campaign for compensation was followed by 'is tassie going to get there own team?!?!' -.-

2 notes · View notes

missnoirr · 2 years

Text

the fact that f1 executives or w/e can say that women are only interested because they want to fuck the drivers even tho it’s just cars going around on a track. and the afl threw a fit about being called the aflm but think it’s outrageous that the aflw wants to drop the w. and that the nhl said that bubbles would help players bc they wouldn’t be distracted by women. and that female sports journalists have to ask better questions while wearing better outfits but not so much better that it might be construed as wanting to fuck the athletes like. you can’t win. it will never be enough. if you are one of the boys or you are outrageously feminine or nothing at all you will always be a letter on the end of a system that hates you.

#you cannot win you will never win and when you do they will change the rules to exclude you #f1 #nhl #I’m pretty sure that’s not my hockey tag but I can’t remember what it is fjjdbdhdf

8 notes · View notes

lupinepublishers · 2 years

Text

Lupine Publishers|Molecular detection of aflatoxin producing Aspergillus species isolates in some chicken meat cuts in Gharbiya governorate, Egypt

Abstract

Contamination with fungi and their toxins is considered one of the most dangerous hidden pollutants that threaten the health of the consumer. The presence of mycotoxins in various foods has been recorded, despite their apparent safety for human consumption. Therefore, the current study was conducted to evaluate the prevalence of Aspergillus species by culture method; and aflatoxinproducing genes molecularly in total of 75 random samples of chicken cuts represented by wing, breast and thigh (25 of each) that were collected from various groceries and poultries shops located at Gharbiya governorate, Egypt. Results of culture and isolation techniques revealed detection of Aspergillus sp. in 36, 48 and 44% of the examined wing, breast and thigh samples, respectively. Moreover, microbiological identification of the isolated strains showed presence of A. niger, A. flavus, A. fumigatus, A. terreus and A. parasiticus in 16, 13.3, 10.6, 1.3 and 1.3% of the total population of the examined samples. Molecular detection of some aflatoxin production regulating genes (OmtA, Nor1 and Ver1) in ten Aspergillus sp. isolates revealed their detection in 8/10 (80%), 8/10 (80%) and 7/10 (70%) represented by positive bands at molecular weight of 1024, 400 and 537 bp, respectively. Referring to the recorded results, chicken cuts may possess a great silent hazard to the human being under improper good manufacturing practices and inadequate hygienic conditions during handling and storage.

Introduction

Chicken meat and meat products production in developing countries plays an essential role in supporting food security and poultry meat demands Wong et al. [1]. Contamination of meat products with molds can be occur during different preparation stages during slaughtering under bad hygienic conditions using contaminated water or by adding contaminated spices with mold spores or during packing, handling, transportation and storage Khalalfalla et al. [2]. Contamination of meat with Aspergillus species, especially Flavi section, is one of the most hazardous microbial contamination as the majority of Aspergillus species are able to aflatoxins production that can cause diseases associated with aflatoxin poisoning and carcinogenic effects Leggieri et al. [3]. Acute aflatoxin poisoning may lead to death as was recorded in Kenya in 2004 Probst et al. [4], while chronic poisoning may lead to various recorded mutagens and cancers Benkerroum [5]. Aspergillus sp. was classified into two groups depending on their toxigenic impacts on food and human health; 1st group includes the aflatoxigenic species such as A. flavus and A. parasiticus, while the 2nd group contains the non-aflatoxin-producing species such as A. tamarii and A. oryzae Frisvad et al. [6]. Molecular analyses have been used to confirm aflatoxin productivity of Aspergillus species isolates. omtA, nor1 and ver1 genes are from the commonly used genes encoded aflP, aflD and aflM toxins detection in food items Sohrabi and Taghizadeh [7] yield an accurate, rapid and reliable records of toxigenic aspergillus species especially in food chain Sadhasivam et al. [8]. Therefore, the main target of the current study was to investigate the presence of toxigenic aspergillus species in some chicken meat cuts collected from Gharbiya Governorate markets, Egypt.

Material and Methods

Collection of samples : A total of seventy-five random samples of raw chilled chicken wing, chicken thigh, chicken breast (25 of each) was collected from different local poultries shops and different supermarkets at Gharbia governorate, Egypt. Samples were taken aseptically in polyethylene bags and were transferred to the laboratory in ice box for mycological examination. Preparation of samples (ISO [9]): Twenty-five grams from each sample were carefully and aseptically homogenized in blinder after mixing with 225 ml of sterile peptone water 0.1% to form a dilution of 1:10, from which tenth fold serial dilutions were prepared.

Determination of Aspergillus species

Culture of the prepared samples was performed according to ISO [10], where 0.1ml of the previously prepared serial dilutions was spreaded by mean of sterile L-shape glass rod over two Petridishes contained solidified Dichloran Rose Bengal agar with chloramphenicol (DRBC) then were incubated at upright position at 25Oc for 5 -7 days. Identification of isolated strains was performed according to Pitt and Hocking [11] macroscopically and microscopically as recorded in Table 1. Molecular detection of some aflatoxin producing genes of some isolated Aspergillus strains by cPCR

Oligonucleotide primers used in cPCR

Three pairs of omtA, nor1 and ver1 primers were prepared and collected from Metabion (Germany). Their special sequence and amplify certain products as were be displayed in Table 2.

Mycological DNA was extracted following QIAamp DNeasy Plant Mini kit Catalogue no. 69104. Preparation of master mix and thermal profile was adapted according to the manufacturer instructions (Emerald Amp GT PCR mastermix (Takara) Code No. RR310A).

Results

As recorded in Table 3, Aspergillus sp. was detected in 32(42.6%) of the total examined samples. In detail, breast samples recorded the highest contamination level (48%); followed by thigh and wing samples, respectively. Regarding with the genus identification, A. niger had the highest detection levels (16%) in the examined samples (Table 4). Referring to the obtained results of molecular detection of some aflatoxin producing genes as recorded in (Table 5) and (Figures 1-3); omtA, nor1 and ver1 genes were detected in 8/10 (80%), 8/10 (80%) and 7/10 (70%) of the examined A. flavus isolates, respectively. presence of these genes indicated the producibility of the examined strain for aflatoxins P, D and M, respectively.

Discussion

Chicken meat and meat products comply an important source of human protein supplement all over the world because they provide good source of digestible protein, low cholesterol fat, essential amino acids, minerals, and different types of vitamins and minerals. In Egypt, as well as human population increasing, demand of animal proteins also is increasing represents a serious challenge in which poultry industry plays an essential role in filling nutrition gap as a rapid and more economic source of proteins (Shaltout et al. [12]). Mold contamination of meat and meat products have been considered a serious source of food spoilage resulting in different organoleptic changes in flavor, color, texture, odor referred mainly to the fungal deterioration especially in poor developing countries due to lack of hygienic measures during processing and handling (Lorenzo et al. [7]). Presence of mold in foods may be referred to the rapid, easy disperse and wide spread of the fungal spores which are abundant in the environment introducing food chain through dust, water, workers and equipment. Their presence in food samples is a serious public health concern as these fungi may be associated with the production of mycotoxins (Benedict et al. [13]). Aspergillus species represents an important mycotic infection in public health concern as a human pathogen and as toxin-producing food contaminant. It releases a lot of spores which found in air, water, soil, plant debris, manure and animal feed. As fungal spore’s growing, it secretes digestive enzyme and mycotoxins leading to food spoilage and human mycotoxicosis (Richardson and Rautemaa-Richardson [14]). Referring to the recorded results in Table 3, Aspergillus sp. was prominently detected in breast samples other than wings and thighs samples, which came in agree with the previously recorded results of Darwish et al. [15] and Shaltout et al. [16] who found that the examined breast samples were more contaminated with fungal infection than wing and thigh samples. While the current prevalence of aspergillus species in the examined samples came lower than those recorded by Hassan [17] who found Aspergillus sp. in all the examined samples (100%) collected from Gharbiya governorate, Egypt. Moreover, Abuzaid et al. [18] also detected A. flavus and A. niger in 40 and 80% of the examined sausage samples of chicken origin, respectively. Referring to the obtained results of the microbiological identification of Aspergillus sp. isolates as recorded in Table 4, they came in agree with the previously reported results by Darwish et al. [15] who found that A. niger was the predominant detected strain, followed by A. flavus and A. parasiticus in the examined samples of chicken cuts collected from Zagazig city, Egypt. Some mold species can cause respiratory infections representing a significant risk for individual with severely weakened immune system (OSHA [19]). Presence of mold in high incidence indicate bad hygienic measures adopted during handling, preparation and processing El Abbasy [20]. Mycotoxins have been defined as naturally occurring secondary fungal metabolites produced in meat and meat products by direct growth of toxigenic molds such as Aspergillus species which produce Aflatoxins and Ochratoxins which threat public health due to their carcinogenic, hepatotoxic, nephrotoxic, teratogenic and mutagenic effects in human and animals Agriopoulou et al. [21]. Aflatoxins are produced by a polyketide pathway that pass through about twenty-seven enzymatic reactions which have been regulated by sets of genes including nor-1, ver-1 and omtA have been shown to be involved in this process. aflD (nor-1) encodes a norsolorinic acid ketoreductase needed for the conversion of the 10-keto group of Norsolorinic Acid (NOR) to the 10-hydroxyl group of Versicolorin A (VERA) Zhou and Linz [22]. aflM (ver-1), predicted to encode a ketoreductase, is involved in the conversion of VERA into Sterigmatocystin (ST) Henry and Townsend [23]; aflP (omtA) codes for O-methyltransferase, which is one of the main genes responsible for transforming ST into O-methylsterigmatocystin (OMST) that is the precursor for aflatoxin production Yabe et al. [24]. Many other previous studies recorded detection of these genes in their Aspergillus isolates of food origin by various PCR techniques; Manonmani et al. [25], Rodrigues et al. [26], and Hassan et al. [27], who conducted several studies investigating the aflatoxigenicity of Aspergillus sp., could detect different genes in their Aspergillus isolates [28,29].

Conclusion

It could be concluded that, breast samples revealed the highest contamination levels with Aspergillus sp.; in addition, A. niger was the prominently detected strain. PCR technique is a unique diagnostic tool for detection and identification of aflatoxigenic Aspergillus strains especially if the field of food safety. So, application of strict hygienic measures, proper use of water supply and food additives from good sources is recommended.

https://lupinepublishers.com/research-and-reviews-journal/fulltext/molecular-detection-of-aflatoxin-producing-aspergillus-species-isolates-in-some-chicken-meat-cuts-in-gharbiya-governorate-egypt.ID.000255.php

For more information about The Research and Reviews on Healthcare: Open Access Journal Archive page click on below link https://lupinepublishers.com/research-and-reviews-journal/archive.php For more information about lupine publishers page click on below link https://lupinepublishers.com/index.php

#lupine publishers #lupine publishers group #lupine publishers LLC #the research and reviews on healthcare: open access journal #rrhoaj

2 notes · View notes

aflm · 2 years

Photo

Page 445 and the first page of Chapter 16 are just about finished after a long day working it out yesterday. Looking like a 2 page week! Volume 1 of Solarion Sun is now available for purchase at aflm.gumroad.com Read Solarion Sun for free at SolarionSun.com with a new page every Friday. Subscribe at patreon.com/aflm for even more pages than the free website! #art #artist #digitalart #lineart #comic #comicbook #indiecomic #webcomic #independentartist #character #characterart #characterartist #characterdesign #thundercats #fantasy #fantasycomic #fantasyart #fantasyillustration #dnd #dungeonsanddragons #brother #sister #twins #family #adventure #patreon #newpage https://www.instagram.com/p/CfXB7mpuUHo/?igshid=NGJjMDIxMWI=

2 notes · View notes

t-jfh · 2 months

Text

Adelaide’s Mark Keane holds his head after being concussed by Port’s Sam Powell-Pepper.

(Photo: Getty Images)

A concussed Nathan Murphy before he was subbed out of the 2023 grand final.

(Photo: Paul Rovere)

Angus Brayshaw. (Photo: Getty Images)

The burning question that no one dares to ask when it comes to concussion in sports

For more than 20 years, every new concussion in footy and every premature retirement is described as a wake-up call for the sport, so either we’re wide awake now and complacent still, or we need a new alarm clock. At very least, we’ve got to stop hitting the snooze button.

The cycle can be broken only by answering an existential question that no one is prepared to ask. When it comes down to it, what toll would be considered acceptable? In a contact sport, the answer cannot be zero, but beyond that, no one knows where to draw the line or what drawing it might mean for the code.

By Greg Baum

The Age - February 28, 2024

•

Demons player Joel Smith is the latest AFLM player to be caught up in a drug scandal.

(Photo: Getty Images)

AFL players take cocaine just like a million other Australians. Get over it.

Opinion by Brandon Jack

The Age - February 28, 2024

•

#Sport #AFL football #Contact sport: risks & injuries: head concussion #Sport players & drug use #Drug & substance abuse #Sports doping regulations & players protection

0 notes

fazcinatingblog · 27 days

Text

Freo winning games just feels right and I'm so glad they've rejoined the competition after their hiatus in 2023

#aflm

3 notes · View notes

nickthetoaster · 1 year

Text

AFLM Predictions - Round 1, 2023

Time to dust off the cobwebs again for another year of the AFLM, and we’ll try and have a bit more nuance and thought into our discussion posts. Not much to say for the start of the year, though; Geelong’s rating is massively higher than anyone else’s and them going back-to-back looks very likely (although we did say the same about Melbourne last year). On the back-end, we’re building our…

View On WordPress

0 notes

b3crew · 5 months

Text

Duck Amuck in Japan: Episode 06 | Home For the Holidays | B3 - Boston Bastard Brigade

The Duck Amuck in Japan gang gives thanks to a new episode, even though King Baby Duck's Thanksgiving food selections are very, very slim. Our Japan resident stumbles upon a secret Banksy shop close to his apartment, and AFLM's favorite band finally announces a US Tour! THE CHERRY COKE$ rock Shimokitazawa, and a day of rakugo and manzai comedy surprises KBD. Plus, the team looks at Suzume and Scott Pilgrim Takes Off, and how things are shaping up this Christmas season!