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#toluidine blue
fruitsclipper · 3 months
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also i have no merch of prompto ffxv but i still love him so much. its been like. Its going to be Holy fuck.. 8 years in like september. whaat the fuck theres no way. number one fictional guy of all time to me. Number two actually. shadow the hedgehog is cooler. i had a crush on him when i was 9 i think.
Whatever im going to bed i have work in the morning ^_^ setting up samples of blue and also um. making standards for the data collection. whee! goodnight lol 💜
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Hello, Mr. Holmes! How are you?
So, long story short, I ended up with an optical microscope in my room more or less 4 months ago, with 200 previously made slides (secured in a proper box), and lots of new ones too, for me to prepare myself. I love microbiology (it's one of my hyperfixations, curse my neurodivergency) and now I love it even more (my mother has had to drag me away from the microscope - I named it Wesley - in the middle of the night multiple times now).
After much conversation, I finally convinced my mom to buy me the proper equipment to prepare the slides!
So, I'm sending this ask to you, as I know you also have a microscope and that you use it a lot: what kind of equipment do you recommend me buying (gloves, scalpel blades, tints, etc), while still remembering that all of the stuff needs to stay in my room (properly taken cared of by me, of course)?
For example, I'm unsure if different dyes are used for different smears and specimens due to it's affinity (I've noticed that on 'organic matter' slides, images are usually tinted purple or pink, while on plant-based slides, images are usually tinted green and blue, with a few red structures.) Considering that I don't have access to a mortuary, I will mostly make plant slides. There must be a difference in the dyes then, right?
Sorry for the long text! Hope this isn't too much of a bother.
- a 17-year-old :)
Congratulations on your new light microscope. I do hope you get the best out of it. I am overjoyed that someone else appreciates the art of microscopy and microbiology.
However, you need to be careful to not strain your eyes. It is recommended to take breaks every 15 minutes to close your eyes or focus on something in the distance to reaccommodate your eyes. And get up every 40 minutes, stretch and correct your posture. And it is recommended to not use a microscope more than 5 hours per day. John has to chase me away from my microscope sometimes to take a break when I sit there for hours, my posture like a Caridea.
Concerning equipment, you will obviously need a scalpel or other sharp blade to make very thin slices of your specimen, as thin as possible. And forceps to move your samples (best just get a whole dissection kit it has everything). Obviously slides and coverslips, pipettes for the stains or water, maybe some tubes. A pen to label your slides. In many staining procedures ethanol or acetone is also used. A waste jar to safely dispose of any chemicals, but be careful what you mix. A rack for staining and containers. I would recommend nitrile gloves, some people are sensitive to latex.
The dyes you use depend on the specimen. For example in histological slides of tissues hematoxylin and eosin are most commonly used (short HE-stain). That's what you most likely saw on your slides, it's blue, purple and pink. Hematoxylin is a basic compound extracted and oxidised from the logwood tree (Haematoxylum campechianum), and it stains acidic compounds in the cells (or basophilic because they have an affinity for basic substances). For example nucleic acids like DNA or RNA get stained by hematoxylin because they are basophillic. And where are lots of nucleic acids? In the nucleus and ribosomes, that is why they appear blue to purple in the staining because they bind hematoxylin. Eosin is an acidic compound, and stains basic or acidophilic compounds red or pinkish, like proteins, collagen, cytoplasm, extracellular matrix.
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(Ductus epididymidis with HE-stain)
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(Tongue HE-stain, pointer marking a ganglion; that is my picture)
Of course there are more specific stains for specific tissues like Golgi's silver staining for neurons.
For plants toluidine blue is often used, high affinity for acidic tissues, and can stain blue to green to purple. It is often combined with safranin, a basic azine, which is probably the red stain you saw. It stains polysaccharides and lignin, woody parts of the plant. Safranin and astrablue is also often combined, astrablue stains non-lignified parts of the plant.
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(Ulex europaeus stem; not my pictures I don't have any samples currently, source Atlas of plant and animal histology)
Safranin is also used in bacteriology, in the famous Gram staining. In Gram staining you use crystal violet (blue/purple), Lugol's iodine solution, then wash it with ethanol and add safranin (red) as a counter stain. Bacteria is gram-positive if the crystal violet stays in their thick murein cell wall, can't be washed out with the ethanol and the bacteria stays blue. Gram-negative appear red because of the counterstain.
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(Staphyloccocus aureus (violet, gram positive) & Escherichia coli (red, gram negative); not my picture, source Wikipedia)
However, I am not sure whether you have access to any of those substances, if they are too expensive for you or if they are too hazardous if used in your own room for a prolongued time. Of course those substances need to be stored properly, and your own room is probably not a good place, especially for ethanol or acetone. The fumes. I would recommend to ask your biology or chemistry teacher whether they can recommend anything further and where to buy said solutions in your area, and if they can't they are idiots. There are also many useful resources and tutorials on Youtube.
Another fascinating experiment for your microscope, that you can perform without buying any chemicals, is a hay infusion. You put hay into a container filled with water, and let it sit undisturbed for a week in a sunny area but not in direct harsh sunlight. During that time the microorganisms in the hay are reproducing in the solution, feeding on the polysaccharides of the hay. Protozoans also flourish in the hay infusion and eat the bacteria. It might get cloudy and a bit foul smelling (best not do it in your own room if you don't want to sleep next to a rotting smell). When you put a drop of the solution onto a slide and look at it in the microscope, you should see a variety of microorganisms like bacteria (like Bacillus subtilis), amoeba, ciliates, heliozoa, algae et cetera. At different depths of the liquid you should find different kinds of organisms, because of differing oxygen content. However, pathogens can also occur in the hay infusion so handle it carefully and work sterile, wash your hands properly.
And even if you don't work at a morgue you can still get tissue samples to experiment on, after all meat is sold in supermarkets, basically the same as a human body. And at the butchers they even sell organs like chicken hearts, pig kidney, liver, blood et cetera. Or observe your own hair under the microscope.
Which kind of samples and slides were included in your starter kit? Be careful to not leave them lying around in the sunlight, or the stain might fade. Always store them in the proper box.
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drjamesjwoytash · 8 months
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A Beginner’s Guide to Histology
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Histology is the study of tissues. As an essential field of medical science, histology is used to diagnose and treat diseases, understand the anatomy and physiology of different organs, and develop new therapies. This branch of science is crucial in medical diagnosis, scientific research, forensic investigation, and archaeological studies.
Medical professionals, including pathologists, diagnose diseases like cancer and infections using histology. By studying tissues at a cellular level, histologists help bridge the gap between anatomy and physiology, providing valuable insights into tissue behavior and reactions to treatments.
The human body has four main types of tissues: epithelial, connective, muscle, and nervous tissue. Epithelial tissue covers the body surfaces, lines the organs and cavities, and serves as a barrier to protect against physical, chemical, and biological threats. Connective tissue supports and anchors the organs, stores and transports nutrients and waste, and participates in immune responses.
Muscle tissue contracts and relaxes to generate movement and force. It is classified into three types: skeletal, cardiac, and smooth muscle. Nervous tissue transmits and processes information through specialized cells called neurons and glia, forming the complex nervous system network.
Histological analysis involves essential steps to prepare tissues for microscopic examination. The process includes fixing the tissue to prevent decay, processing and embedding the tissue in supporting material, sectioning it into thin slices, and finally, staining the sections to highlight specific structures. Various stains are available, each chosen to enhance the visibility of certain components within the tissues.
The most common and widely used stain is the Hematoxylin and Eosin (H&E) stain, which colors cell nuclei blue and cytoplasm pink. Toluidine blue, Wright's stain, and trichrome stains are other techniques that produce different colors to reveal specific structures or proteins within the tissues.
Pathologists carefully examine tissue samples obtained through biopsies or autopsies, identifying abnormal cells and providing valuable information for treatment planning. This helps doctors make accurate diagnoses and develop appropriate treatment strategies.
In the field of research, scientists utilize histology to investigate tissues at a cellular level, contributing to advancements in medicine, biology, and genetics. The detailed examination of tissues aids in understanding various diseases and unlocking potential therapeutic targets.
Histology also leaves its mark on forensic science. In unexplained deaths, microscopic analysis of tissues from autopsies provides critical clues, helping forensic experts unravel the circumstances surrounding such incidents.
Beyond the present, histology also aids in exploring our past. In archaeology, the study of ancient tissues, like bones and teeth, sheds light on the lives of past civilizations and species. These insights provide valuable knowledge about our evolutionary history.
Not limited to human health, histology also plays a vital role in veterinary science. Veterinarians rely on histology to diagnose animal diseases accurately, allowing for timely and appropriate treatments. Additionally, histology helps assess the effects of treatments and monitor animal health.
Finally, in the fascinating world of plants, plant histology, also known as plant anatomy, reveals the microanatomy of plant tissues. By studying these tissues, researchers gain insights into plant growth and development, which can affect agriculture and environmental conservation.
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mitivy · 11 months
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CAS NO.101-11-1 N-Ethyl-N-(3'-Sulfo) Benzylaniline supplier /High quality/Best price/DA 90 DAYS
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Quick Details Product name:N-Ethyl-N-benzylaniline-3'-sulfonic acid CAS: 101-11-1 Molecular formula:C15H17NO3S Molecular weight:291.37 EINECS No.:202-916-8 Appearance:Powder or liquid Other names:N-Ethyl-N-benzylaniline-3'-sulfonic acid;4-Amino-2-nitrodiphe; N-Ethyl-N-benzylaniline-3'-sulfonic acid; alpha-n-ethylanilinotoluene-3-sulphonicacid; 3-benzenesulfonic acid; 3--Benzenesulfonicacid; 3-(N-Ethyl-N-phenylaminomethyl)benzenesulfonic acid; 3-{Methyl}benzene-1-sulfonic acid Port: any port in china Packing:  according to the clients requirement Storage: Store in dry, dark and ventilated place. Transportation: by sea or by air payment methods: L/C, T/T, D/A, D/P, O/A, paypal, western union etc.accept all payment. Application It is used as an intermediate in the production of food dye Brilliant Blue. Superiority 1.supply sample 2.the packing can be according the customers` requirment 3.any inquiries will be replied within 24 hours 4.we provide commerical invoice, packing list, bill of loading, coa , health certificate and origin certificate. if your markets have any special requirements, let us know. 5.factory price. 6.prompt delivery. we have good cooperation with many professional forwarders, we can send the products to you once you confirm the order. 7.we can accept various payment methods, l/c, t/t, d/a, d/p, o/a, paypal, western union etc., and we cooperate with sinosure many years. Anyway ,if you need any chemicals from China ,MIT -IVY INDUSTRY CO.,LTD can help you. Company Information MIT-IVY INDUSTRY CO.,LTD is a manufacturer and exporter of fine chemical dyes & pharmaceutical intermediates in China. Mainly produce aniline series products ,chlorine series products,and epoxy curing agent We are a  company full of vitality. The company has a group of energetic, well-trained employees and strong technical research and development capabilities. We specialize in the production, development and sales of API intermediates, fine chemicals and plant extracts. Relying on advanced equipment and strict management, adhere to the business philosophy of "openness, tolerance, innovation, and sharing" to create a win-win cooperationplatform.Everything comes from innovation, it is our philosophy ! If you are interested in getting more quotations, please add WHATSAPP:0086-13805212761 or E-MAIL:[email protected] FAQ Q1:Will you supply samples for testing? A: For most of our products, samples are available, but please cover the shipping cost. Q2:What's your MOQ? A: For the high value product, our MOQ starts from 10g,100g and 1kg. Q3:Which kind of payment terms do you accept? A: Proforma invoice enclosed with our bank information will be sent after confirmation of order. payment methods: L/C, T/T, D/A, D/P, O/A, paypal, western union etc.accept all payment. Q4:How about your delivery time? A: Generally, it will take 3 to 5 days after receiving your advance payment. Q5:How do you treat quality complaint? A:First of all, our quality control will reduce the quality problem near to zero. If there is a quality problem caused by us, we will send you free goods for replacement or refund your loss. Main products Mit-Ivy is a well-known fine chemicals and pharmaceutical intermediates manufacturer with strong R&D support in China. Mainly involved Aniline, Chlorine products. Payment:DA 60 DAYS TEL:008619961957599   E-MAIL:[email protected] 产品 Product CAS   N-甲基间甲苯胺 N-Methyl-M-Methylaniline 696-44-6 N-羟乙基苯胺 N-(2-hydroxyethyl)-Aniline 122-98-5 N-乙基对甲苯胺 N-ethyl-p-toluidine 622-57-1 N,N-二甲基邻甲苯胺 N,N-Dimethyl-o-toluidine 609-72-3 N-甲基邻甲苯胺 N-Methyl-o-methylaniline 611-21-2 N,N-二乙基对甲苯胺 N,N-Diethyl-p-toluidine 613-48-9 N,N-二乙基间甲苯胺 N,N-diethyl-m-toluidine 91-67-8 N-氰乙基-N-羟乙基间甲苯胺 N-cyanoethyl-n-hydroxyethyl-m-toluidine 119-95-9 N-乙基间甲苯胺 N-ethyl-m-toluidine 102-27-2 N-氰乙基-N-羟乙基苯胺 N-cyanoethyl-n-hydroxyethyl aniline 92-64-8 N-乙基邻甲苯胺 N-ethyl-o-toluidine 94-68-8   N,N-二羟乙基对甲苯胺 N,N-dihydroxyethyl-p-toluidine   .3077-12-1   N,N-二乙基苯胺 N,N-diethyl aniline 91-66-7 N-丁基-N-羟乙基苯胺 N-butyl-n-hydroxy aniline 3046-94-4 N-乙基-N-氰乙基间甲苯胺 N-ethyl-n-cyanoethyl-m-toluidine 148-69-6 N-丁基-N-氰乙基苯胺 N-butyl-n-cyano aniline   61852-40-2 N-甲基- N-羟乙基苯胺 N-methyl-n-hydroxyetjyl aniline   93-90-3 N,N-二丁基苯胺 N,N-dibutyl aniline 613-29-6 N-乙基-N-氰乙基苯胺 N-ethyl-n-cyanoethyl aniline   148-87-8 N-正丁基苯胺 N-Phenyl-N-butyl aniline 1126-78-9   N-乙基-N-羟乙基苯胺 N-ethyl-n-hydroxyethyl aniline 92-50-2 N-乙基-N-苄基间甲苯胺 N-ethyl-n-benzyl-m-toluidine 119-94-8 N-甲基-N-苄基苯胺 N-methyl-n-benzyl aniline 614-30-2 N-异丙基苯胺 N-isopropy aniline   768-52-5   N-乙基-N-苄基苯胺 N-ethyl-n-benzyl aniline 92-59-1 N-环已基苯胺 N-Cyclohexylaniline 1821-36-9   N,N-二甲基间甲苯胺 N,N,3-trimethyl- Dimethyl-m-toluidine   121-72-2   N-甲基甲酰苯胺 N-Methylformanilide 93-61-8   N-甲基-N-羟乙基对甲苯胺 N-(2-HYDROXYETHYL)-N-METHYL-4-TOLUIDINE 2842-44-6 N,N-二甲基对甲苯胺 N,N,4-trimethyl-;dimethyl-4-toluidine; Dimethyl-p-toluidine 99-97-8   N-甲基对甲苯胺 N-Methyl-p-toluidine   623-08-5 N,N-二甲基苯胺 N,N-dimethyl aniline 121-69-7 N,N-二羟乙基苯胺 N,N-dihydroxyethyl aniline 120-07-0 N-乙基-N-羟乙基间甲苯胺 N-Ethyl-N-Hydroxyethyl-M-Toluidine 91-88-3   N,N-二羟乙基间甲苯胺 N,N-dihydroxyethyl-m-toluidine   91-99-6   N-乙基苯胺 N-ethyl aniline 103-69-5   N-甲基苯胺 N-methyl aniline 100-61-8 N-甲基对甲苯胺 4-Methyl-N-methylaniline 623-08-5 N-甲基-N-羟乙基苯胺   2-(N-Methylanilino)ethanol 93-90-3 N,N-二甲基对苯二胺 N,N-DIMETHYL-P-PHENYLENEDIAMINE 99-98-9 3-(甲氨基)甲苯 3-(Methylamino)toluene 696-44-6 N,N-二异丙醇对甲苯胺 DIPROPOXY-P-TOLUIDINE 38668-48-3 N,N-二乙基邻甲苯胺 N,N-DIETHYL-O-TOLUIDINE 606-46-2 N-甲基对硝基苯胺 N-Methyl-4-nitroaniline 100-15-2 N,N-二苄基苯胺 N,N-DIBENZYLANILINE 91-73-6 N-苯基乙醇胺 2-Anilinoethanol 122-98-5 N-苄基苯胺 N-Phenylbenzylamine 103-32-2 N-羟乙基间甲苯胺 N-2-HYDROXYETHYL-M-TOLUIDINE 102-41-0 N-乙基N氯乙基间甲苯胺 N-ETHYL-N-CHLOROETHYL-M-TOLUIDINE 22564-43-8   N,N-二乙基-4-氨基-2-甲基苯甲醛 4-Diethylamino-2-methylbenzaldehyde 92-14-8 间甲苯胺 M-Toluidine  MT 108-44-1 1,4-二溴-2,5-二碘苯 1,4-DIBROMO-2,5-DIIODOBENZENE 63262-06-6 N,N-二羟乙基对苯二胺硫酸盐 N,N-Bis(2-hydroxyethyl)-p-phenylenediamine sulphate 54381-16-7 N-乙基-N-苄基-4-氨基苯甲醛 4-(N-Ethyl-N-benzyl)amino-benzoaldehyde 67676-47-5 N,N-二乙基-4-氨基苯甲醛 4-Diethylaminobenzaldehyde 120-21-8 对二甲胺基苯甲醛 p-Dimethylaminobenzaldehyde 100-10-7 2-氨基噻唑 2-Aminothiazole 96-50-4 对甲苯胺 P-Toluidine  PT 106-49-0 N,N-双(2-羟基丙基)苯胺 N,N-BIS(2-HYDROXYPROPYL)ANILINE 3077-13-2 N-乙基-N-氰乙基苯胺 3-Ethylanilinopropiononitrile 148-87-8 N-乙基-N-(3'-磺酸苄基)苯胺 N-Ethyl-N-benzylaniline-3'-sulfonic acid 101-11-1 邻苯甲酰苯甲酸甲酯 Methyl 2-benzoylbenzoate 606-28-0 对羟基苯甲酸甲酯 Methylparaben 99-76-3 十四酸异丙酯 Isopropyl myristate 110-27-0 棕榈酸异丙酯 Isopropyl palmitate 142-91-6 邻甲苯胺 O-Toluidine  OT 95-53-4 4-甲基-N-苯基苯胺 N-PHENYL-P-TOLUIDINE 620-84-8 N,N-二甲基苄胺 N,N-Dimethylbenzylamine  BDMA 103-83-3 N,N-二甲基甲酰胺   N,N-Dimethylformamide  DMF .68-12-2 N-甲基甲酰胺 N,N-Dimethylformamidedimethyl acetal  (DMF-DMA) 4637-24-5 N,N-二甲基乙酰胺 N,N-Dimethylacetamide   DMAC 127-19-5 N,N-二乙基间甲苯甲酰胺 避蚊胺 N,N-diethyl-m-toluamide    DEET 134-62-3 N,N-二乙基羟胺 N,N-Diethylhydroxylamine  DEHA 3710-84-7 N,N-二甲基-间甲基苯胺 N,N-DIMETHYL-M-TOLUIDINE 121-72-2 N-甲基二苯胺 N-Methyldiphenylamine 552-82-9 N,N-二氰乙基苯胺 N,N-Dicyanoethylaniline 1555-66-4 N-乙基-2-硝基苯胺 N-Ethyl-2-Nitro-Benzenamine 10112-15-9 N-(2-羟乙基)乙二胺 AEEA 111-41-1 二乙烯三胺(DETA) Diethylenetriamine  DETA 111-40-0 三乙烯二胺 Triethylenediamine 280-57-9 三乙烯四胺 TriethylenetetramineTETA 112-24-3 四乙烯五胺 TEPA 112-57-2 间二氯苯 1,3-Dichlorobenzene   MDCB 541-73-1 间二三氟甲苯 1,3-Bis(trifluoromethyl)-benzene 402-31-3 粉末丁腈橡胶 MITIVY33-1(POLYMER/ADHESIVE COMPOUNDING) 9003-18-3 十六烷基氯化吡啶 Cetylpyridinium chloride monohydrate 6004-24-6 对氯甲苯 4-Chlorotoluene 106-43-4 无水硫酸钠 SODIUM SULFATE 7757-82-6   /15124-09-1 碱性嫩黄 Auramine O 2465-27-2 偶氮二异丁腈 2,2'-Azobis(2-methylpropionitrile) 78-67-1   Read the full article
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Best Gynecologist Doctor in Vanasthalipuram – Evyahospitals
Obstetrics and Gynaecology
We offer a wide range of treatments in the Department of Obstetrics & Gynecology at Evya Hospital the Best Gynecology Hospital in Vanasthalipuram, from yearly preventative women’s checkups to sophisticated medical and surgical care of women during their reproductive and post reproductive years, including urogynecology care. We deliver first-rate medical care while acknowledging the psychological and interpersonal elements of gynecologic issues and offering the assistance required in these areas. Gynecologists, obstetricians, radiation oncologists, medical oncologists, surgical oncologists, and specially trained gynecologic nurses and social workers make up the highly skilled, multidisciplinary treatment team that works with patients. The treating team includes the patient, the patient’s family, and the referring doctor.
We provide young women with proper, preventative gynecologic care who are worried about menstrual irregularities, contraceptive technologies, and stress. We offer counselling and therapeutic treatment for middle-aged women going through the peri- and post-menopause as they finish their families. When medicinal treatment is ineffective, we provide cutting-edge treatments, such as same-day laparoscopic surgery and trans cervical procedures to remove fibroids and control bleeding.
From common pelvic pain and period-related issues to more serious conditions like endometriosis, fibroids, ovarian cysts or ovarian cancers, or uterus-related issues.
Our team of Best Gynecologist Doctor in Vanasthalipuram at EVYA Hospitals accurately diagnoses and treats a wide range of medical conditions that exclusively affect women. These conditions range from fungal or viral infections, common pelvic pain, and period-related issues.
We also do both modern and traditional hysterectomy operations as well as treat menopause, early pregnancy symptoms, and infertility disorders.
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We provide diagnostic methods to assess female incontinence and medicinal and surgical treatments to enhance pelvic floor and urine function. The department performs abdominal and vaginal operations such vaginal suspension and urinary sling procedures. We provide a range of abdominal, laparoscopic, and transcervical operations, such as the removal of fibroids and ovarian cysts, and we also treat endometriosis and irregular, heavy vaginal bleeding in women.
Best Gynecologist Doctor in Vanasthalipuram provides a wide range of healthcare services that fulfil the needs of women through all the stages of life, starting from pre-conception planning and pregnancy to menopause and beyond.
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Our doctors frequently perform the following procedures and surgeries: adhesiolysis, cervical biopsies, colposcopies, dilation and curettage (D&C), endometrial ablation, uterine or endometrial biopsies, fluid-contrast ultrasound (FCUS), hysterectomy, hysterosalpingography, hysteroscopy, myomectomy, oophorectomy, pelvic ultrasound, pelviscopy, uterine suspensions, uterine (artery) fibroid embolization (UFE) and vulvectomy, vaginal vault, trachelectomy, toluidine blue dye test.
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shermanfanning62 · 1 year
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Review in the quality, oxidative standing as well as diet power valuation on fats employed in non-ruminant pet eating routine.
grams., KEGG). A possible downside of these knowledge-driven approaches could be that the result can be opinionated towards known responses, as a result strategies do not be the cause of the potential for which include novel nutrients, alongside the responses they will catalyze. Results: Below, all of us explore the contrary of increasing bio-mass generate inside three product bacteria, particularly Bacillus subtilis, Escherichia coils, along with Hordeum vulgare, through the use of modest, chemically doable system adjustments. We all utilize the forecast as well as experimentally verified growth prices from the wild-type systems while research beliefs and see the result of inserting mass-balanced, thermodynamically doable tendencies on prophecies of rate of growth by using fluctuation equilibrium analysis. Conclusions: Although many replacements involving current reactions effortlessly create a decrease as well as total loss of bio-mass manufacturing potential check details , in all about three researched bacteria we find feasible alterations which usually facilitate a substantial rise in this particular biological operate. Many of us target alterations together with achievable chemical attributes as well as a substantial surge in biomass deliver. The final results show little modifications tend to be enough for you to substantially adjust biomass generate within the a few creatures. The strategy may be used to forecast the consequence of precise alterations about the generate of any group of metabolites (at the.gary., ethanol), hence offering a computational platform for manufactured metabolism executive. (H) 2012 Elsevier Munster Limited. Almost all protection under the law set aside.Cosmetic transplantation has recently been provided to people with severely disfigured faces not open to be able to functional remodeling along with autologous tissue. Reasons out of this treatment consist of hazards of immunosuppression, the concern that it'll be achieved regarding cosmesis, the point that appropriate renovation can be achieved with autologous tissues, the opportunity impact on the person regarding graft failing, along with the issue that the treatment could possibly be used by simply unqualified centres. Whenever we look for bioethics to help people dissect the issue associated with regardless of whether facial hair loss transplant must be done, we view that most the actual reasons have already been proscriptive as an alternative to prescriptive. You'll find valid quarrels for facial hair transplant in line with the prescriptive pillars involving bioethics: independence, the law, as well as beneficence. This short article attempts to reframe the actual conversation in the prescriptive way.A manuscript antibacterial content (L-PET) ended up being cooked by immobilizing e-polylysine upon polyethylene terephthalate (Dog) nonwoven materials. Floor alterations in the textile had been carried out by by using a compound customization treatment exactly where carboxyl organizations were geared up on the Dog floor, a new combining broker had been grafted, as well as the e-polylysine had been immobilized. Deciphering electron microscopy (Search engine optimization) was adopted to investigate the outer lining morphology with the textiles, whilst the toluidine blue approach and also X-ray photoelectron spectroscopy (XPS) were utilized to gauge your grafting densities. Your anti-bacterial pursuits in the L-PET ended up looked at using the shaking-flask technique.
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devinebowman83 · 1 year
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Large pH Strain Affects Root Morphology and Health Standing regarding Hydroponically Developed Rhododendron (Rhododendron spp.).
In addition, since PEDF will be recently defined as the regulator regarding glucose and fat metabolic rate, we also discuss PEDF structures especially in connection with insulin-sensitizing and also triglyceride-reducing attributes.PDZ area scaffold healthy proteins are designed for putting together macromolecular protein processes inside various mobile processes by means of PDZ-mediated presenting into a quick peptide fragment on the carboxyl tail associated with focus on healthy proteins. How every single PDZ website exclusively acknowledges their targeted protein(utes) remains a serious visual query, because no less than several out of your hundreds PDZ websites in each eukaryotic genome reveal the overlap golf presenting components with a targeted health proteins. Below, we all demonstrate that the domain-swapped dimerization associated with zonula occludens-1 PDZ2 creates a distinct software that will characteristics alongside the well-separated canonical carboxyl tail-binding pocket in each PDZ product throughout binding for you to connexin43 (Cx43). Many of us further demonstrate that the actual charge-charge conversation network shaped through deposits in the PDZ dimer software and upstream deposits of the Cx43 peptide not merely provides the unheard of connection uniqueness for the intricate but may in addition function as a phosphorylation-mediated regulation move to the character in the Cx43 difference junctions. Finally, we offer data Y-27632 in vitro that this sort of domain-swapped dimer set up can also happen inside various other PDZ website scaffolding proteins. Therefore, each of our findings found a new paradigm for understanding how a few PDZ domain meats particularly situation to as well as get a grip on your characteristics of their goal proteins.Introduction: Ankylosing spondylitis (AS) is different in its pathology where swelling starts on the entheses prior to advancing to a osteoproliferative phenotype generating too much bone enhancement that may lead to shared mix. The underlying components of the development are generally inadequately understood. The latest function offers recommended which modifications in Wnt signalling, a key bone regulatory process, may give rise to combined ankylosis inside While. While using proteoglycan-induced spondylitis (PGISp) mouse product which shows spondylitis and also ultimate combined fusion pursuing an initial inflamation related stimulus, we've got recognized the actual structural along with molecular changes that underlie condition progression. Methods: PGISp mice were recognized 3 months soon after introduction associated with irritation making use of histology, immunohistochemistry (IHC) as well as term profiling. Results: Infection begun with the outside from the intervertebral dvds moving on to disk destruction then enormously excessive normal cartilage as well as bone tissue matrix development, as exhibited through toluidine blue soiling and IHC regarding collagen kind My spouse and i along with osteocalcin, leading to syndesmophyte creation. Appearance amounts of DKK1 and SOST, Wnt signalling inhibitors extremely portrayed in important joints, have been decreased by simply 49% and also 63% correspondingly within the spinal column PGISp weighed against manage rodents (G < Zero.05) along with SOST self-consciousness validated by simply IHC. Microarray profiling showed genes associated with swelling as well as immune-regulation were transformed.
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webs-of-life · 1 year
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Mickley, James. Pinus contorta (Shore Pine) Needle Cross Section, Stained with Toluidine Blue. Micrograph. Corvallis, December 23, 2021. Oregon State University.
100x magnification on Zeiss Primostar compound microscope for Plant Structure class. 
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Oral Pathology
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If you think that you may have a dental cancer, an appointment with an oral pathologist can aid you find the very best therapy for the disease. Early diagnosis is important, and also 90 percent of dental cancer situations can be successfully treated when caught early. Oral pathologists utilize cutting-edge technologies to detect oral cancer cells, including toluidine blue, or "T-Blue," which is swabbed onto thought lesions. The resulting reaction will certainly determine whether a biopsy is required to establish the root cause of the cancer cells. Dental pathology can be tricky to identify, especially when symptoms are nonexistent. Despite the fact that dental lesions are not generally unpleasant, they can have major effects, which can make your life significantly less pleasurable. 
In order to figure out the cause of your mouth abscess, an oral pathologist will certainly accumulate your medical history and examine your mouth. The symptoms can be light or severe, depending on the sort of infection. Often a mouth abscess signifies a more serious problem, such as gum condition or a cancer. The most typical oral sores consist of recurring herpes labialis, aphthous stomatitis, and candidiasis. While these problems prevail, they can likewise signify a wider problem, such as immune deficiency. In fact, some of the much more usual diseases can be detected with biochemical pens, including staphylococcal disease as well as autoimmune illness. In the medical field, dental pathology is a field of dentistry that deals solely with the diagnosis and therapy of diseases of the mouth as well as maxillofacial region. Visit this article source to discover more about oral implants now.
The area entails microscopic, biochemical, and radiographic evaluations. Oral pathologists additionally supply biopsy solutions to dental professionals and also provide medical medical diagnosis based upon the results of the microscopic and histologic exams. Usual dental conditions that oral pathologists deal with consist of mouth cancer, mumps, salivary gland disorders, microbial infections, and also a lot more. Oral pathology is an integral part of dental care. A dental pathologist can diagnose and also deal with dental problems swiftly. Oral illness, such as gum condition, are a major reason for tooth loss, as well as a failure to diagnose or treat them can cause tooth loss. The oral field incorporates various dental concerns, including genetic variables. And also oral pathologists are the best physicians to help clients with these concerns. If you are willing to find out more about the subject, read the article linked here.
And, of course, you can count on the medical professionals at Accuracy Dental surgery to assist you preserve a healthy and balanced smile. In addition to diagnosing oral cancer, oral pathology can additionally diagnose a selection of noncancerous irregularities. Herpes simplex infection, as an example, is the reason for fever blisters, an excruciating, red or white place on the lip. The infection is likewise in charge of the cold aching as well as fever blister. With timely treatment, oral pathology can avoid this destructive disease. It is necessary to be attentive regarding oral exams because you might have a problem that requires a dental consultation. Another usual problem impacting the mouth is mucocele, a repeating cyst located in the mucosa. 
This benign development generally happens on the lower lip, buccal mucosa, or the floor of the mouth. Although it can be existing at any type of age, one of the most usual time to diagnose a mucocele remains in the third decade of life. The person will have difficulty speaking and swallowing, however the good news is, oral surgery can fix it with marginal scarring. For more enlightening info about this topic, visit this site: https://en.wikipedia.org/wiki/Pathology.
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dinigudem · 2 years
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Akva vital bedienungsanleitung philips
  AKVA VITAL BEDIENUNGSANLEITUNG PHILIPS >> DOWNLOAD LINK vk.cc/c7jKeU
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https://dinigudem.tumblr.com/post/692060492465848321/bedienungsanleitung-sigma-sport-bc-500-altic, https://xeluqoneru.tumblr.com/post/692060813673054208/esx-quantum-qe-9004-bedienungsanleitung-yamaha, https://lekewonujuca.tumblr.com/post/692060818711396352/lifetec-mikrowelle-md-14482-bedienungsanleitung, https://dinigudem.tumblr.com/post/692060492465848321/bedienungsanleitung-sigma-sport-bc-500-altic, https://suvisujigo.tumblr.com/post/692060590545485824/medion-lifetab-md-99200-bedienungsanleitung-7490.
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nancyclerk74 · 2 years
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Examination with the Cytotoxicity regarding Peptide Improvements of Rilmenidine Phosphate in Tetrahymena pyriformis
0001). The suggest AFP Mommy wasn't drastically distinct involving the installments of acrania as well as instances of spina bifida (One particular.81 as opposed to. 1.Seventy-five; g Is equal to Zero.722). The particular discovery costs involving NTD inside testing by serum AFP had been 50.0% (95% C-list 31.9-68.A single) and Thirty eight.5% (95% C-list Twenty one.1-56.Several) from repaired false-positive rates involving Ten and 5%, correspondingly. Conclusion: Dimension of maternal dna solution AFP with 11-13 weeks' gestation could be valuable in screening process pertaining to baby NTDs. Trademark (Chemical) This year Azines. Karger AG, BaselTarget. People with fibromyalgia syndrome (FM) report several physical, cognitive, as well as subconscious signs and symptoms. The objective of the current study ended up being determine whether people with FM differed in line with the variety and also seriousness of signs, and if so, whether or not subgroups vary regarding medical care consumption, functional capacity, as well as function position. Methods. Symptom, medical care utilization, function, and actual files ended up intended for Only two,182 woman responders to an Internet survey. Element evaluation had been performed #Link# about the actual and cognitive/psychological symptoms #Link# , along with resulting factor scores were utilized in a bunch evaluation to distinguish subgroups based on signs or symptoms. Chaos groups have been in contrast on a list of specifics (at the.grams., healthcare usage, problem management). Results. Factor looks at led to 3 indicator factor standing: orthopedic, non-musculoskeletal, along with cognitive/psychological. symptoms. The perfect bunch solution to the actual group analysis unveiled Several groups. Group A single ended up being on top of all 3 symptom internet domain names, team A couple of had been average for the 2 actual indicator internet domain names and high about cognitive/psychological signs or symptoms, group Three or more was #Link# moderate on the Only two actual indicator domains and low about cognitive/psychological signs, and party 4 was have less just about all symptom websites. The harder systematic teams documented the maximum amount of medical care usage and also trouble in handling signs. Conclusion. Your FM human population is heterogeneous with regard to indicator confirming. Additional scientific studies are necessary to far better recognize differential symptom expertise between people who have FM. Rationalization of such differences may possibly increase understanding of the particular components involved with FM and provide advice with regard to treatment method judgements.The past due preterm infant was created 4.5 l right after intraamniotic injection associated with Ninety days mg involving Toluidine blue to verify premature rupture involving walls. Due to baby exposition for the dye, the human body in the patient has been azure tarnished along with the baby suffered with methemoglobinemia, Heinz' system optimistic hemolytic anemia and also hyperbilirubinaemia necessitating exchange transfusion. These issues underline which antenatal exposition of toluidine glowing blue may result in sizeable postnatal infant morbidity.
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fruitsclipper · 5 months
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i should do geo chemistry in the lab only. no equations just give me 2 molar sodium hydroxide and ill figure it out from there 👍 science talk below the cut im just blabbering
i think the question im trying to figure out for work is if pH has any effect on ars dye like, falling out of suspension/ precipitating. because even in the samples ive filtered (so theyre liquid only) it happens and i have to show my professor so he knows im not fucking with the data like Help.... a quarter of a gram heated dolomite and like 6mM ars and its precipitating all over the place. Theres like a layerrr on top of the dolomite its so weird. i think we should also use the actual real magnesium/ calcium oxide powders too and see how they compare bc theyre Supposed to be the same as the heated minerals (dolo -> MgO + calcite / calcite -> CaO) maybe. maybe not. idk. Any way sorry again proessor i dont really know how to do the equations. i know we have the midterm on thursday im trying 👍 im really trying . Please help re: the lab experiment i dont know why it does that. Also!!! the fucking toluidine blue dye keeps leaving these blue rings in the glass vials. idk if it affects anything its just weird blue ring... peofessor pleae ....... Sience 🧪 ..... in non serious terms the i thinj. dolomite and tb dye makes a pretty color. it doesnt really do the best at removing the dye which is what we want (dyes are sort of like a stand-in for aqueous contaminants, the clearer the end liquid is the better) but it makes such a pretty purple color . Whatever. stop chasing perfection dude. Just go to sleep if u dont finish the assignments its not the end of the world <- reminder to self. If you read this hi. sorry if itdoesnt make sense its almost 2am here. i love you 💕
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photospl · 2 years
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Tulip Pollen (Toluidine Blue)
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phenolph-thalein · 4 years
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Histological stains
Basic dyes react with anionic or acidic components in cells. Nucleic acids are acidic, and therefore bind to basic dyes.
Methyl green - stains green
Methylene blue - stains blue
Pyronin G - stains red
Toluidine blue - stains blue
Acidic dyes react with cationic or basic components in cells. Proteins and other components in the cytoplasm are basic, and will bind to acidic dyes.  Another way of saying this is that cytoplasmic proteins are acidophilic (acid liking - i.e. bind to acidic dyes).
Acid fuschin - stains red
Aniline blue - stains blue
Orange G - stains orange
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laurelnose · 4 years
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for my own purposes: a series of chemistry notes on adapting the ingredients for the post-Industrial Revolution process of clearing and staining for a medieval universe:
DISTILLED WATER: i don’t know why i thought this was a later process but we’ve been distilling water for forever, lmao
95% ETHYL ALCOHOL: high-proof alcohols are very canon
“CLEARING” AGENT: these days we use trypsin, and while maybe magic could make isolating a digestive enzyme from the pancreas and processing it into a shelf-stable powder in the middle ages plausible MY suspension of disbelief would sure be broken. prior to widespread use of trypsin you could use caustic potash (KOH) or lye (NaOH) for tissue maceration instead. using lye probably has adverse effects on the success rates (there’s a reason we use trypsin and even so, you still get a lot of disintegrated specimens—lye is probably worse but again the actual numbers for how many specimens just straight-up melt do not exist, so who fucking knows) but it would be very possible. KOH is used in conjunction with trypsin today and has been used on its own in the past; it’s slightly on the modern side compared to lye but it’s actually really easy to make—slaked lime (dates at least to 1600s) + regular potash (K2O3, produced in Temeria in Season of Storms) precipitates CaCO3 & produces KOH in solution, you just have to filter out the carbonate.
BONE STAIN (ALIZARIN RED S): alizarin is one of two red dyes in madder (the other is purpurin). we’ve had madder forever and we’ve been aware of its bone-staining properties since at least the 1500s. technically we only separated alizarin out of madder in the 1800s but regular madder will still stain calcium and i don’t think having purpurin mixed in would affect a specimen since purpurin doesn’t seem to have any particular affinity for tissues, so i can either handwave that they figured out how to isolate alizarin a few hundred years early or say they used madder whole.
CARTILAGE STAIN (ALCIAN BLUE): super difficult and toxic to produce actually. i didn’t know this but it certainly explains why many early (late 1800s/early 1900s) and amateur c+s specimens are only stained with alizarin! methylene blue & toluidine blue can also stain cartilage but they aren’t any easier to produce with pre-industrial techniques. there’s a red and a yellow dye that will bind with cartilage but not only are they similarly difficult to produce, to contrast the bone stain red and yellow are useless. (side note: for some terrestrial species you have to remove the fat properly or alcian blue won’t penetrate & for that the recommended substance is acetone. i do not know how acetone is produced but if i’m not using alcian i don’t have to explain acetone.) verdict: you really could not do cartilage staining pre-industrial revolution, unless you want to make up something involving magic. NERVE STAIN (SUDAN BLACK B): i understand none of the industrial dye words used around this substance. this is probably a no go (i don’t even know if you CAN do nerve stains for full-body (i.e. not histological) specimens other than fish when it seems a lot of terrestrial specimen prep involves manually removing a lot of the flesh?) but i think nerve staining is cool as hell and deserves more attention in all the romanticization of the alizarin/alcian specimens, so, i’m going to keep looking FINAL STORAGE MEDIUM: c+s specimens are basically always stored in glycerol because it matches the refractive index of cleared tissue and makes the flesh appear totally invisible, although you will get some heated arguments about what percent of the solution should be glycerin and what percent regular alcohol. glycerin wasn’t actually discovered until 1779, but it’s a byproduct of soapmaking so technically it would have been pretty accessible. it could perhaps have been isolated earlier if someone was looking; what i’m unsure of is the quality/purity of the glycerin that could be achieved—you really need it to be clear as glass. you CAN just store C+S specimens in EtOH—it doesn’t have as good a refractive index but it will technically work fine, specimens would just be less transparent. on the other hand, the trials and tribulations of working with glycerin are emotionally important to me. further research needed
ANTIBACTERIAL SUBSTANCE (THYMOL): this is derived from plants—specifically, thyme, hence, thymol. it was first isolated in 1719 but i have yet to figure out how that was done historically—today we do some intensive industrial processing to produce it at scale, which isn’t feasible. however, they could just use the plant—poultices of other thymol-containing plants have antiseptic properties even if they’re not pure, & at this stage in the process (i.e. the very end) i think the worst effect of using the plant directly would perhaps be some staining of the storage medium. it would definitely not be ideal in a modern scientific context where we want to control all our chemical variables but i think it would work. however, if i’m not using glycerin it probably isn’t really necessary because its purpose is to prevent stuff from growing in the glycerin and most things do not grow in 95% EtOH.
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baifuchem · 3 years
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Description Brand: BaiFuChem Product Name: m-Tolyldiethanolamine Synonyms: m-Tolyldiethanolamine, N,N-Dihydroxyethyl-m-toluidine CAS No: 91-99-6 MF: C11H17NO2 MW: 195.26 Packaging: 25 kg/ fiber drum,or according to the requirements of the customers Application 1. Used as an intermediate for solvent red 117, disperse violet 33, 40, 43, disperse blue 96,
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