#Spectometry

[uicscience]

Like many octopuses, the California two-spot octopus declines and dies shortly after breeding. (Tom Kleindinst, Marine Biological Laboratory)

Changes in cholesterol production lead to tragic octopus death spiral

While octopuses are known for their intelligence and unique ability to change color and regenerate limbs, they also often suffer a tragic death: after laying eggs, mothers stop eating and die. Sometimes, they speed up death by mutilating themselves and twisting their arms into a tangled mess.

The source of this maternal behavior seems to be the optic gland, an organ similar to the pituitary gland in mammals. For years, just how this gland triggered death was unclear, but a new study by researchers from the University of Illinois Chicago, the University of Chicago, and the University of Washington shows that the optic gland in maternal octopuses undergoes a massive shift in cholesterol metabolism, resulting in dramatic changes in the steroid hormones produced. 

Alterations in cholesterol metabolism in other animals, including humans, can have serious consequences on longevity and behavior, and the study’s authors believe this reveals important similarities in the functions of these steroids across the animal kingdom, in soft-bodied cephalopods and vertebrates alike.

The study findings are published in Current Biology. 

Co-authors Stephanie Cologna, associate professor of chemistry at UIC, and Melissa Pergande, a former graduate student at UIC, who specialize in mass spectometry, analyzed the chemical composition of samples produced by the maternal octopus optic gland.

They found three different pathways involved in increasing steroid hormones after reproduction. One of them produces pregnenolone and progesterone, two steroids commonly associated with pregnancy. Another produces maternal cholestanoids or intermediate components for bile acids, and the third produces increased levels of 7-dehydrocholesterol (7-DHC), a precursor to cholesterol.

The new research shows that the maternal optic gland undergoes dramatic changes to produce more pregnenolone and progesterone, maternal cholestanoids, and 7-DHC during the stages of decline. While the pregnancy hormones are to be expected, this is the first time anything like the components for bile acids or cholesterol have been linked to the maternal octopus death spiral. 

The findings suggest that disruption of the cholesterol production process in octopuses has grave consequences, just as it does in other animals. So far, the team has discovered another step in the octopus self-destruct sequence, signaling more changes downstream that ultimately lead to the mother’s odd behavior and demise.

The study, “Steroid hormones of the octopus self-destruct system,” was supported by the National Science Foundation, UIC, the Illinois Board of Higher Education, and the Marine Biological Laboratory. 

This story was adapted from a University of Chicago news release written by Matthew Wood. 

[studyingchemeng]

27.05.20 I 3/100 days of productivity

Had the first online lab today and you bet I was wearing my lab coat as I was watching 😂 and yes my handwriting gets progressively worse and messier as I am taking notes!

#studyblr #studyspo #study motivation #studying #study notes #days of productivity #chemical engineering #100 days of productivity #uni studyblr #uni student #uni studyspo #uni #university #uniblr #Lab #Chemistry #Analytic chem #Spectometry

[sanyuktak]

#protein sequencing market #healthcare research report #market analysis #allied market research #mass spectometry #edman degradation

[imperium-romanum]

“A USF professor rewrote a tasty part of Italian history after running tests on the inside of a 4,000 year-old jar found in a prehistoric settlement in Castelluccio, Italy.”

“The jar in question was found in the 1990s by archaeologist Giuseppe Voza. According to Davide Tanasi, since it was located in the center of a hut, it was clearly of some relevance.”

Continue reading

#classics #tagamemnon #tagitus #history #ancient history #italy #ancient italy #jar #artefact #artifact #material culture #culture #giuseppe voza #davide tanasi #Institute for Digital Exploration #idex #gas chromatography #mass spectometry #nuclear magnetic resonance #research #examination #olive oil #Ioannis Gelis

[thewhisperingdoc]

June 2, 2020

I am going to meet my friend-cum-sister tomorrow at work after 2 months.. and I am pretty excited 🙂 She has always been a real inspiration throughout my life... and I look upto her each day!

Slightly disturbed after watching the news today..

I studied about spectometry today all the time humming filmy songs in my mind... so yeah, not so much of a concentrated study 😐

The picture above is a cosy place at my home which I like... once I think I saw the International Space Station on a clear starry night from that window...and I am not lying 🤔

Day 17/100 days of productivity

#windows #100 days of productivity #my work #friends #inspiration #news #studyblr #filmyhit #quarantine life

[silentauroriamthereal]

Ah, writing Holmes-universe fanfic: the only hobby that would otherwise require me to be googling how mass spectrometers work at 12:21am. I now also possess a great deal of unrelated information that I never asked for, such as where to buy them, a goodly number of types of other forensics lab equipment, how forensic autopsies work, and about how long to expect various mass spectometry tests to last. Fun fun.

Fic’s over 13,000 words and growing steadily. :)

#wee update #writer stuff #things I never needed to know: this #sherlock #johnlock

[babygirlbraids]

I would unironically love to hear more about your research proposal?? It sounds fascinating and I'm hoping to go into neuroscience myself so this is right up my alley. How did you get into this field? What's your research methodology like? What are its real-world applications? Sorry if this is super upfront, I'm just really interested in your research!

Oh my God I was going through my asks and suddenly realised I completely forgot to answer this. Anon I’m so sorry I hope you’ll still read this. Okay so I can’t say too much because this research will actually be carried out at some point but I’ll try to go into detail as much as possible. This will probably be long so I’m going to put it under a cut.

The research project was part of a course called Research Methods I took last period. So what we want to do is see if dopaminergic axons lead serotonergic axons to the forebrain, since it’s a very important neurotransmitter system. The method can be divided into three aims:

1. Use TRAP-sequencing to label mitochondria in dopaminergic neurons, pull them out of the brain and do mass spectometry to identify proteins that are produced in the dopaminergic neurons. 

2. Match the found proteins to mTOR pathway, a pathway that’s important for dopaminergic axonal growth.

3. Create genetic knockout mice where we remove the genes that code for the identified and matched proteins. There’ll be wildtype mice, heterozygous knockouts and homozygous knockouts.

TW: Use of mice as test animals. 

We will then cut out the prefrontal cortex of the brains of mice in the early postnatal stages. The mice will be tested for medial prefrontal cortex innervation, using immusostaining for the dopaminergic and serotonergic axons. The brains will also be sent out for HPLC-analysis, so we can see the effect the gene removal has had on dopamine/serotonin neurotransmitter balance.

Remaining mice will be allowed to grow into adults and will be used for behavioural testing, where tests related to autism spectrum disorder will be used to asses any behavioural deficits. The brains of these mice will then be analysed in the previous ways to check for any long lasting connectivity and neurotransmitter issues. 

The results will be relevant to quite a lot of neurodevelopmental disorders (autism, major depressive disorder, schizophrenia, etc) since they involve dopamine/serotonin neurotransmitter imbalances. 

I’m so sorry for taking so long to reply, anon. I really hope you got to read this!!

#ask

[spectometry-blog]

autism recovery,biomedical treatment for autism,autism help for parents,treatment options for autism,autism solutions

[sp3ec7to5met-blog]

[james3090-blog]

 Spectometry  offers a biomedical system for helpings kids recovery from autism spectrum  disorder.

[georgefomitchev]

All you need to know about LIBS/LIPS spectroscopy Check the material components with Aurora 4000 Spectrometer and an Endurance 10 watt DPSS laser

https://youtu.be/XIqOydb-Awk

#spectometry #spectroscopy #lips #libs #laser #laserinduced #power #materialcheck #endurance #endurancelasers

[lectio-divina]

Ciclo - Ristampa delle Storie Brevi N.137 (Ritardatari 2)

(immagine via Tumblr/spectometrie)

« Mgn, mgn, » Faceva la vecchia arcigna avvolta nella sua stola di pelliccia, consultava il tabellone alto in fondo alla sala, che era un catino di un'ampiezza spropositata, oltrechè quasi completamente deserto, e dichiarava a sé stessa di andare per due, il 23 e il 33. « Mgn, mgn, » Rosicava. Si guardò attorno con aria circospetta per valutare lo stato d'animo dei suoi competitori. « 23! » Gracchiò l'altoparlante in fondo alla sala, lei avvampò: « Mgn, mgn, » Riprese a fare, ora a una frequenza più elevata, dato che andava per uno, le venne di andare al gabinetto, ma incrociò le gambe e resitette stoica. « 32. » Riprese l'altoparlante suadente, « Eeeeee!! » Esclamò qualcuno alle sue spalle, lei si volse, in tempo per vedere il tripudio di un fossile minuscolo con gli occhiali a fondo di bottiglia, che sventolava in aria le sue schede. « Baldracca. » L'appellò lei, ricambiandola minacciosa.

Piaciuta la storia?

Compra il libro.

Cane Bianco, di Fester Abrams, un giallo, lo trovi su Amazon , gratis con Kindle Unlimited, da Giunti e da Feltrinelli.

Manhattan, Battery Park, pomeriggio, una giovane donna porta il cane a passeggio, un incontro fortuito, un attimo di leggerezza e il cane scompare. Sera, lobby del Sagram Building, Upper East Side, “Il quartiere delle calze di seta”, il padre della giovane, persona avanti negli anni, ma determinato al limite del coriaceo, esce di casa deciso a ritrovare il cane. Farà l'alba sui marciapiedi, fra personaggi grotteschi e onirici, baristi dei bassifondi e derelitti da strada, ciascuno avrà in mano una traccia, una moneta da scambiare col ricco signore, chi per gioco o empatia, chi per invidia o disprezzo. Sono ore di febbrile ricerca, che condurranno il protagonista verso un finale inaspettato: Che ne è stato del cane bianco?

Un giallo imprevedibile, su cui abbiamo lavorato a lungo, tutti insieme, riversandoci le nostre cure, attente e appassionate, sia per la sostanza che per la forma. Il risultato è una gioia, per gli occhi e per la mente, un piacere che soltanto una piccola casa editrice è in grado di offrire.

Edito da Lectio Divina edizioni - LOVE AND READ BOOKS -

lectio-divina.tumblr.com ©Tutti i diritti riservati

#iorestoacasaeleggo #letturatime #letturachepassione #letturaconsigliata #letturaveloce #letturatura #polizieschi #librigialli #romanzodiracconti #gialli #romanzi #lacasadicarta #librichepassione #instalibri #librimania #letturaestiva #letturadellevacanze #ioleggoacasa #leggere #storiebrevi #libridaleggere #leggerefabene #libri #racconti #lacasadicarta #librochepulsa #casalettori #lettoricompulsivi #leggeresempre #bookstagramitalia #librodelgiorno #polizieschi #librigialli #romanzodiracconti #romanzi #instalibri #librimania #ioleggoperche #libriamoci #libriconsigliati #raccontibrevi #anni #bhfyp #booklover #ioleggoperche #libriamoci #scrittura #leggereovunque #libriconsigliati #raccontibrevi #librichepassione #storiebrevi #storie #ioleggo

[ecoamerica]

Watch the American Climate Leadership Awards 2024 now: https://youtu.be/bWiW4Rp8vF0?feature=shared

The American Climate Leadership Awards 2024 broadcast recording is now available on ecoAmerica's YouTube channel for viewers to be inspired by active climate leaders. Watch to find out which finalist received the $50,000 grand prize! Hosted by Vanessa Hauc and featuring Bill McKibben and Katharine Hayhoe!

#ACLA24 #ACLA24Leaders #youtube #youtube video #climate leaders #climate solutions #climate action #climate and environment #climate #climate change #climate and health #climate blog #climate justice #climate news #weather and climate #environmental news #environment #environmental awareness #environment and health #environmental #environmental issues #environmental justice #environment protection #environmental health #Youtube

[laurenmansfieldkl]

Critical Reflection: Hepatitis B Screening Project

As apart of our 2 month internship here at Neogenix, we must be involved in the Hepatitis B Screening Project carried out by Neogenix every year. This is a project that involves every single public primary school in Malaysia, Neogenix receives samples from the students and we run the tests to confirm a positive/negative result for each student. Our roles as interns is to not only receive the specimen and carry out pre-processing but we also carry out the tests itself. Unlike the other diagnostic tests that are run by senior lab technicians, the Hepatitis B Screening Project allows interns to carry out the testing. This involves well-plates and spectometry and is quite a long process and includes distributing samples into wells, making sure controls wells are placed, washing steps, spectrometry and stop solution. Normally it will take a whole day to get the results from a single batch as the process is quite rigorous. Nethertheless, this opportunity has been incredibly eye-opening, interesting and has allowed us interns to receive a more practical hands-on learning experience. The sheer volume of samples has been challenging and we often have to leave later depending on when we receive samples. The name checking and assigning lab numbers to each sample is also challenging, as some batches have incorrect spelling of names and missing numbers for which we have to refer back to the school and confirm student names. Dr. Yew our supervisor has advised that we can come up with any approaches to this process that may be able ease the efficiency of processing these samples. Overall, I’m extremely grateful for the opportunity to be apart of a project that I can learn a lot from. 

[immundnzltd]

We develop bioanalytics methods for #pharmacokinetic and #toxicokinetic studies of drug molecules in #preclinicalresearch. We use mass spectrometry & immunoassays (ELISA) and mass spectometry-based assay pAMS to help you discover innovative medicines.

#Immundnz #immunologyCRO

[thoothukudipeople]

கீழடி நாகரிகம் 2600 ஆண்டுகள் பழமையானது: இந்திய வரலாற்றையே மாற்றும் அகழ்வாய்வு முடிவுகள்.. மதுரை அருகே உள்ள கீழடியில் மேற்கொண்ட தொல்லியல் ஆய்வில் கிடைத்த பொருட்களை ஆராய்ந்ததில் தமிழக சங்ககாலம் என்பது மேலும் 300 ஆண்டுகள் பழமையானது எனத் தெரியவந்திருப்பதாக தமிழகத் தொல்லியல் துறை தெரிவித்துள்ளது. மதுரை நகரத்திற்கு தென்கிழக்கில் சுமார் 15 கிலோ மீட்டர் தூரத்தில் சிவகங்கை மாவட்டத்தில் அமைந்திருக்கும் கீழடி கிராமத்தில் 2014ஆம் ஆண்டில் மத்திய தொல்லியல் துறை நடத்திய ஆகழ்வாய்வில் அங்கு, 2,000 ஆண்டுகளுக்கு முற்பட்ட காலத்திலிருந்து மனிதர்கள் வாழ்ந்ததற்கான ஆதாரங்கள் கண்டுபிடிக்கப்பட்டன. இந்தப் பகுதியில் மேலும் ஆய்வுகளை மேற்கொள்ள கீழடி அகழ்வாய்வு கண்காணிப்பாளர் அமர்நாத் ராமகிருஷ்ணா விண்ணப்பித்திருந்த நிலையில், அவர் அங்கிருந்து அசாமுக்கு இடமாற்றம் செய்யப்பட்டார். இந்த நிலையில், அங்கு அகழ்வாய்வைத் தொடர மாநில தொல்லியல் துறை முடிவுசெய்தது. அதற்குப் பிறகு மாநில தொல்லியல் துறை சார்பில் அகழ்வாய்வு தொடர்ந்து நடைபெற்றுவந்தது. அந்த அகழ்வாராய்ச்சியில் கிடைத்த பொருட்கள் மீது செய்யப்பட்ட ஆய்வின் முடிவுகள் தமிழக தொல்லியல் துறையால் வெளியிடப்பட்டுள்ளன. வியாழக்கிழமை தமிழ் வளர்ச்சித் துறை அமைச்சர் மஃபா பாண்டியராஜன், தொல்லியல் துறை செயலர் த. உதயசந்திரன் ஆகியோர் இதனை வெளியிட்டனர். 2018ம் ஆண்டில் கீழடியில் தமிழக அரசால் நடத்தப்பட்ட 4வது அகழ்வாய்வில் கிடைத்த பொருட்களை வைத்து கிடைத்த முடிவுகள் இதில் தொகுக்கப்பட்டுள்ளன. கீழடி நாகரீகத்தின் காலம் என்ன? கீழடியில் கிடைத்த 6 பொருட்கள் ஆக்சலரேட்டட் மாஸ் ஸ்பெக்ட்ரோமெட்ரி (Accelerated mass spectometry) ஆய்வுக்காக அமெரிக்காவின் ஃப்ளோரிடாவில் உள்ள பீட்டா அனலிடிகல் லேப்பிற்கு அனுப்பப்பட்டன. அதில் கிடைத்த முடிவுகளின்படி, அந்தப் பொருட்கள், கி.மு. மூன்றாம் நூற்றாண்டுக்கும் கி.மு. ஆறாம் நூற்றாண்டுக்கும் இடைப்பட்ட காலத்தை சேர்ந்தவை எனக் கண்டறியப்பட்டுள்ளது. கீழடியில் 353 செ.மீ. ஆழத்தில் கிடைத்த பொருள் கி.மு. 580வது ஆண்டையும் 200 செ.மீ. ஆழத்தில் கிடைத்த பொருள் கி.மு. 205வது ஆண்டையும் சேர்ந்தது எனக் கண்டறியப்பட்டுள்ளது. இந்த இரு மட்டங்களுக்கு கீழேயும் மேலேயும் பொருட்கள் இருப்பதால், கீழடியின் காலகட்டம் கி.மு. ஆறாம் நூற்றாண்டு முதல் கி.மு. ஒன்றாம் நூற்றாண்டு வரையிலானது என தொல்லியல் துறை முடிவுக்கு வந்துள்ளது. #keezhadi #keezhadiexcavation #keezhadicivilization #keezhaditamilcivilization (at Keezhadi) https://www.instagram.com/p/B2tIyTQB-cy/?igshid=1v4i7rrd8hc3c

#keezhadi #keezhadiexcavation #keezhadicivilization #keezhaditamilcivilization

[rosewilsonworth]

Rules: tell your followers eleven facts about yourself and then tag eleven more people to do it.

Tagged by: huntress-earthtwo thank you very much :D

1-  I have three cats

2-  I need to pass 5 subjects in university in order to graduate

3- I'm currently studying the hardest (and most useless for my future) subject and i have no idea what it’s going on (it’s related to spectometry if anyone’s interested)

4- The first comic book i bought was ‘Harley Quinn: hot in the city’. I kinda regret it bc conner/palmiotti are just bad but i’m too emotional to sell it because it was the first comic i bought and all... 

5- The reason i first got into comics was because i thought batwoman and harley quinn looked ‘interesting’. turns out ‘interesting’ was code for gay but i had no idea at the time. but they were also interesting so...

6- I can read tarot cards and the palm of the hand (i don’t know what it’s called in english and i’m too lazy to check)

7- The reason i don’t watch supergirl is bc everyone in the cast (except James Olsen) annoyed me, especially the actresses who played sanvers. when i found out the maggie actress wasn’t a latinx i got pissed off but i felt that my dislike of her was justified 

8- I love art but i can’t create art, i don’t have any talent :(

9-  I cry a lot about anything not just sad movies. once i cried at a museum when i saw a 15th century painting, it sounds pretentious as fck but i had tears running down my face, an old lady offered me a tissue, it was embarrasing

10-  95% of the time I'm perfectionist, 5% of the time i’m like ‘fuck that shit who the fuck cares’

11- I’m currently procrastinating. i need to go back to studying

#thank you once again for tagging me #:D #mina talks #things i was tagged in