#Xylose Lysine Deoxycholate Agar
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Understand the Spiderman of the Microverse: XLD Agar
Learning XLD’s X, Y, and Z can be challenging, but not with us. In the previous article, we discussed the various needs and applications of XLD (click on the link if you haven’t read it). In this, we’ll talk more about its composition, principle, observations, and modifications.
What is the composition of XLD Agar?
It was developed by Welton Taylor in 1965. XLD stands for Xylose Lysine Deoxycholate Agar and is a bright pink or red-colored solid opalescent gel medium. Knowing its key contents will help you better understand its principles and differentiating abilities.
Ingredients g/l
Yeast Extract 3 g
L-lysine 5 g
Xylose 3.75 g
Lactose 7.5 g
Sucrose 7.5 g
Sodium Deoxycholate 1 g
Sodium Chloride 5 g
Sodium Thiosulfate 6.8 g
Ferric Ammonium Citrate 0.8 g
Phenol red 0.08 g
Agar 12.5 g
Distilled water 1 Litre
Yeast extract provides the medium with nutrients, vitamins, peptides, and other essential growth factors. Xylose, lactose, and sucrose are rich sources of fermentable carbohydrates. For the detection of fermentation of these carbs in the medium, a pH indicator, phenol red, is added. The addition of xylose to the medium accounts for the differentiation of various fermenting enteric bacteria from species of Shigella that do not ferment it. Most enteric pathogens, including Salmonella,can ferment xylose, which results in the formation of acidic byproducts and a change in the color of the medium from pink to yellow, whereas Shigella colonies remain red. In some cases, Salmonella imitates Shigella colonies and gives red colonies, which are then differentiated by the production of Hydrogen sulfide gas by the metabolism of thiosulfate and give black centers. Enterobacteria such as E. coli can ferment lactose in the medium.
XLD Agar’s composition can be adjusted according to one’s needs and choices and is hence available in many different variations, but the basic principle remains the same.
Observation and Inferences on XLD Agar:
Observation Inferences
Red colonies, some with black centers Salmonella spp.
Red colonies Shigella spp.
Yellow to orange colonies Coliforms
Pink, flat, and rough colonies Pseudomonas aeruginosa
While learning about XLD Agar, a question that must have run through your thoughts would’ve been, “Where is XLD Agar available?” Let’s look into it.
Where is XLD Agar available?
XLD Agar is available on the market as Dehydrated Culture Media in a number of variations according to one’s requirements, including plant-based and animal-based options.
These Dehydrated Culture Media can be dissolved in distilled water according to the concentration mentioned on the package, autoclaved, poured, and used.
TM Media, the microbiology division of Titan Biotech Ltd., offers a wide range of XLD Agar as Dehydrated Culture Media in a number of variations suitable for all your needs, operations, and specific requirements.
The product range includes the following:
XLD AGAR MODIFIED (as per ISO) TM 1621: XLD Agar, modified, is a selective and differential medium for the isolation of gram-negative enteric pathogens from clinical specimens or food products. It is a modification of the original formulation of Taylor that allows selective isolation of Salmonella typhi, E. coli, Salmonella enteritidis, Salmonella typhimurium, and Shigella flexneri. It is recommended by the ISO committee, and the composition and performance criteria of this medium are as per the specifications laid down in ISO 6579-1:2017.
XLD AGAR (VEG.) TMV 492: Xylose Lysine Deoxycholate Agar (Veg) is prepared by replacing Sodium Deoxycholate with synthetic detergent No.III, which makes the medium free of BSE/TSE risks. Xylose Lysine Deoxycholate Agar (Veg) is suitable for the isolation and identification of enteric pathogens from stool samples.
XLD AGAR (as per USP/EP/JP/BP) TMH 112: This medium is employed for pharmaceutical testing and non-sterile product testing for the detection of Salmonella after enrichment in Rappaport VassialidiasSalmonella Enrichment Broth in accordance with the harmonized method of USP/EP/BP/JP/IP.
XLD AGAR TM 1448: It is used for pharmaceutical testing and nonsterile product testing for the detection (or absence) of Salmonella after enrichment in Rappaport Vassialidias Salmonella Enrichment Broth in accordance with IP.
XLD AGAR TM 492: XLD Agar exhibits increased selectivity and sensitivity as compared to other plating media, e.g., SS Agar, EMB Agar, and Bismuth Sulphite Agar. The media formulation does not allow the overgrowth of other organisms over Salmonella and Shigella. Samples suspected of containing enteric pathogens, along with other mixed flora, are initially enriched in Modified Semisolid RV Medium Base.
Why choose TM Media’s XLD Agar?
TM Media’s superior-quality and diverse Microbiological Culture Media are certified by ISO, CE, GMP, FSSAI, and FSSC.
TM Media provides unrivaled convenience, dependability, and efficacy, as well as versatility and flexibility, by manufacturing a contamination-free medium with a longer shelf life and reproducible outcomes.
Conclusion:
XLD Agar is a selective and differential agar medium primarily used for the isolation and differentiation of different enteropathogenic organisms that are well-known to cause diseases like Food poisoning, Gastroenteritis, and other Digestive illnesses.
By choosing TM Media, consumers invest in precision, quality, and excellence. TM Media's product portfolio includes over 2000 Dehydrated Culture Media, along with Ready-to-Use Culture Media, Biological Media Bases, Media Supplements, Lab Chemicals, and many more.
#XLD Agar#dehydrated culture media#food microbiology#clinical microbiology#Microbiology#Xylose Lysine Deoxycholate Agar
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Get the Best Quality of XLD Agar at TM Media
XLD Agar (Xylose Lysine Deoxycholate Agar) is a selective and differential medium used primarily for isolating and identifying enteric pathogens, particularly Salmonella and Shigella species. It contains xylose, lysine, lactose, sucrose, and the pH indicator phenol red. Salmonella colonies typically appear red with black centers due to hydrogen sulfide production, while Shigella colonies remain red. The medium's selectivity is enhanced by deoxycholate, which inhibits Gram-positive bacteria and some Gram-negative coliforms. XLD Agar is widely used in clinical and food microbiology.
Product link: https://www.tmmedia.in/product/xld-agar-2/
Website link: https://www.tmmedia.in/
Company Name: TM Media
Phone: +91-9999-1687-70
Address: 905, 9th Floor, Big joes Tower, Netaji Subhash Place, New Delhi
Mail:[email protected]
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Get the Best Quality of XLD Agar Composition (TM 492) at TM Media
If you are looking for Xylose Lysine Deoxycholate (XLD) agar (TM 492) manufactured by TM Medium, a distinctive culture medium, boasts a specialized blend of ingredients carefully selected to facilitate the isolation and differentiation of enteric pathogens. Featuring xylose and lactose as fermentable carbohydrates, lysine and thiosulfate as vital sulfur sources, and deoxycholate acting as a potent selective agent against gram-positive organisms, xld agar composition stands out for its precision. Incorporated phenol red serves as a pH indicator, transitioning the medium to a discernible yellow hue upon acidification from carbohydrate fermentation. Additionally, the inclusion of ferric ammonium citrate and sodium thiosulfate facilitates hydrogen sulfide production, distinguished by the formation of characteristic black colonies.
Visit the Website - https://www.tmmedia.in/
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Get the Best Quality of XLD Agar Composition (TM 492) at TM Media
If you are looking for Xylose Lysine Deoxycholate (XLD) Agar manufactured by TM Media stands out as a valuable medium specifically crafted by Taylor for the precise identification of Enterobacteriaceae, a family of bacteria commonly associated with gastrointestinal infections. This specialized agar medium finds its primary application in microbiological testing, particularly in the isolation and differentiation of enteric pathogens. The targeted differentiation includes distinguishing Salmonella Typhi from other Salmonella species commonly found in diverse sources such as foods, water, and dairy products.
The unique formulation of xld agar composition provides heightened selectivity and sensitivity, setting it apart from other plating media like SS Agar, EMB Agar, and Bismuth Sulphite Agar. The distinct advantage lies in its ability to curb the overgrowth of competing organisms over Salmonella and Shigella, offering a more accurate and reliable means of identification.
Visit the Website - https://www.tmmedia.in/
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XLD Agar: Composition, Principle, Uses- TM Media
XLD Agar was formulated by Taylor for the isolation and differentiation of enteric pathogens including Salmonella typhi from other Salmonella species. XLD Agar has been recommended for the identification of Enterobacteriaceae and for the microbiological testing of foods, water and dairy products. The media formulation does not allow the over growth of other organisms over Salmonella and Shigella. XLD Agar is one of the media used in the Microbial Limit Tests in the USP & EP.
PRINCIPLE
The medium contains Yeast extracts as source of vitamins and minerals. Addition of Sodium deoxycholate acts as a selective agent which is inhibitory to Gram-positive bacteria. It suppresses the growth of other enteric pathogens and enhances the growth of only few enteric bacilli. The medium contains Xylose as a fermentable carbohydrate which is utilized by Salmonella species. The medium pH is changed due to fermentation of Xylose which is detected by indicator Phenol red, thus the
colony colour turns red. The medium also contains Lactose and Sucrose as the source of fermentable sugar. L-lysine is an essential amino acid source. Lysine is added to differentiate Salmonella Sp.Sodium chloride helps maintaining the osmotic balance of the cells. This medium also allows differentiation of bacilli based on their ability to produce H2S; it contains Sodium thiosulphate and Ferric ammonium citrate that helps visualizing the black centered colonies on production of hydrogen sulphide in the medium. Agar is added as the solidifying agent.
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XLD Agar For selective isolation and enumeration of Salmonella typhi and other Salmonella species. XLD Agar was formulated by Taylor for the isolation and differentiation of enteric pathogens including Salmonella Typhi from other Salmonella species.
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Get the Best Quality of XLD Agar Composition (TM 492) at TM Media
If you are looking for Xylose Lysine Deoxycholate (XLD) Agar (TM 492) manufactured by TM Media, a specialized culture medium, boasts a unique blend of xylose, lysine, and deoxycholate, meticulously crafted to foster the growth of enteric pathogens. Through its selective inhibition of gram-positive organisms, coupled with the selective fermentation properties of xylose, xld agar composition offers a distinct advantage in differentiating between pathogenic and non-pathogenic bacterial strains. Its characteristic formulation not only facilitates the isolation of Salmonella and Shigella species but also provides a reliable platform for the detection and identification of other enteric bacteria, making it an indispensable tool in clinical diagnostics and microbiological research.
Visit the Website - https://www.tmmedia.in/
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Find the Best Quality of XLD Agar Composition (TM 492) at TM Media
If you are looking for Xylose Lysine Deoxycholate (XLD) Agar )(TM 492) manufactured by TM Media is a meticulous blend of xylose, lysine, deoxycholate, and other select ingredients, meticulously orchestrated to foster the growth and differentiation of Enterobacteriaceae. With its distinctive combination of nutrients and inhibitors, xld agar composition creates an environment that selectively supports the growth of Salmonella and Shigella species while inhibiting the proliferation of other microorganisms. This unique composition, carefully calibrated by microbiologists, serves as a specialized tool in the detection and isolation of enteric pathogens, guiding researchers through the intricate world of clinical microbiology with precision and reliability.
Visit the Website — https://www.tmmedia.in/
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Check the Best Quality of XLD Agar (TM 492) at TM Media
If you are looking for XLD Agar (TM 492) (Xylose Lysine Deoxycholate) manufactured by TM Media, a brainchild of Taylor, stands tall in the realm of microbiology, heralded for its prowess in isolating and differentiating Enterobacteriaceae, notably Salmonella Typhi, from a milieu of pathogens. Crafted with precision, its formulation boasts superior selectivity and sensitivity, outshining conventional plating media like SS Agar and EMB Agar. Notably, XLD Agar's composition thwarts the proliferation of competing organisms, ensuring clarity in pathogen identification. Employed in diverse settings, from food to water analysis, its efficacy remains unrivaled, epitomizing reliability and accuracy in microbiological testing.
Visit the Website - https://www.tmmedia.in/
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Find the Best Quality of XLD Agar Composition (TM 492) at TM Media
If you are looking for Xylose Lysine Deoxycholate (XLD) Agar manufactured by TM Media, a pioneering formulation by Taylor, boasts a unique blend of xylose, lysine, deoxycholate, and other selective ingredients. Tailored for the isolation and differentiation of enteric pathogens, it serves as a stalwart medium in clinical and food microbiology. With its specialized composition, xld agar composition inhibits unwanted microbial growth while fostering the development of distinctive colonial characteristics. Its selectivity and sensitivity surpass conventional plating media, ensuring accurate identification of Salmonella and Shigella species. XLD Agar's precise formulation underscores its pivotal role in microbiological diagnostics and research endeavors.
Visit the Website - https://www.tmmedia.in/
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Get the Best Quality of XLD Agar Composition (TM 492) at TM Media
If you are looking for Xylose Lysine Deoxycholate (XLD) Agar manufactured by TM Media, a harmonious blend of xylose, lysine, and deoxycholate, choreographs a selective symphony for isolating enteric pathogens. This distinctive composition, enhanced by brilliant green and iron salts, creates a stage where Salmonella and Shigella pirouette in vibrant hues, revealing their distinct characteristics. Balancing inhibition and growth promotion, XLD Agar is a performance of precision, providing microbiologists with a nuanced medium to unravel the microbial ballet in diverse samples. From clinical specimens to food diagnostics, the choreography of xld agar composition orchestrates a diagnostic dance, allowing the spotlight to shine on specific enteric actors with elegance and clarity.
Visit the Website - https://www.tmmedia.in/
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Find the Top Quality of XLD Agar Composition (TM 492) at TM Media
If you are looking for xld agar composition (Xylose Lysine Deoxycholate) manufactured by TM Media is a specialized medium consisting of xylose, lysine, deoxycholate, and other selective ingredients. This unique composition facilitates the isolation and identification of specific enteric pathogens from complex microbial samples. The incorporation of xylose and lysine allows for distinctive metabolic reactions, while deoxycholate acts as a selective agent. Together, these components create an environment that encourages the growth of target organisms, aiding in accurate microbiological analyses.
Visit the Website - https://www.tmmedia.in/
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Get the Best Quality of XLD Agar (TM 492) at TM Media
If you are looking for Xylose Lysine Deoxycholate (XLD) Agar manufactured by TM Media is recommended for identifying Enterobacteriaceae and microbiological testing. Developed by Taylor, it isolates and differentiates enteric pathogens, emphasizing Salmonella Typhi. XLD Agar offers heightened selectivity and sensitivity compared to SS Agar, EMB Agar, and Bismuth Sulphite Agar. Its formulation prevents the overgrowth of other organisms, ensuring accurate detection of Salmonella and Shigella in samples suspected of containing enteric pathogens. Initial enrichment is carried out in Modified Semisolid RV Medium Base.
Visit the Website - https://www.tmmedia.in/
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Find the Top Quality of XLD Agar (TM 492) at TM Media
If you are looking for XLD Agar (Xylose Lysine Deoxycholate) manufactured by TM Media is a primary medium, selectively cultivating gram-negative Enterobacteriaceae like Salmonella and Shigella. Developed by Taylor in 1965, it is vital in various fields, including pharmaceutical and environmental testing. With an initial bright pink or red appearance, indicative of a neutral pH around 7.4-7.6, it leverages the fermentation properties of enteric bacteria, especially Salmonella, leading to medium acidification and a color change to yellow. Shigella, not fermenting xylose, remains red, facilitating their differentiation.
Visit Website - https://www.tmmedia.in/
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Check the Best Quality of XLD Agar Composition (TM 492) at TM Media
If you are looking for Xylose Lysine Deoxycholate (XLD) manufactured by TM Media, agar boasts a distinctive composition featuring xylose, lysine, and deoxycholate. This selective and differential medium is specifically formulated for the isolation and identification of enteric pathogens, particularly Salmonella and Shigella species, xld agar composition unique blend inhibits the growth of non-enteric bacteria and encourages the differentiation of bacterial species based on their ability to ferment xylose, produce hydrogen sulfide, and decarboxylate lysine. This medium plays a crucial role in clinical microbiology for the detection and characterization of enteric pathogens in various specimens.
Visit the Website - https://www.tmmedia.in/
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Check the Best Quality of xld agar manufacturer (TM 492) at TM Media
If you are looking for XLD Agar is manufactured by TM Media company following precise formulations. Leading manufacturers adhere to quality standards, ensuring consistent composition. The production involves blending ingredients like xylose, lysine, and deoxycholate to create a selective and differential medium for isolating Enterobacteriaceae. Stringent manufacturing processes guarantee reliable performance in identifying bacterial species based on their biochemical characteristics. Labs and healthcare facilities worldwide trust reputable xld agar manufacturer, a crucial tool in microbiological diagnostics.
Visit the Website - https://www.tmmedia.in/
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