#Mesenchymal Stem Cells

[bpod-bpod]

Building Back Better

You slip, trip or fall and crack...your bone breaks. The bigger the break, the longer it takes to heal, as bone and blood vessels rebuild. Sometimes a bone graft is needed for complete recovery. However, current engineered bone grafts promote bone formation alone. Researchers now present a scaffold that promotes bone and blood vessel formation – microgels containing proteins that mimic the matrix in which bone cells sit. When human mesenchymal stem cells (MSCs) were added to microgels containing chitosan, gelatin and hydroxyapatite, they attached and matured into bone cells. Next, MSCs were added to microgels containing only gelatin. Fluorescence microscopy revealed MSCs attached (pictured, left), stretched out (right) and matured into networks of endothelial cells, which line blood vessels. Combining the two microgels encouraged and enhanced bone and blood vessel formation. This lays the foundation for creating scaffolds that promote both new bone and blood vessels to heal fractures.

Written by Lux Fatimathas

Image from work by Matthew D. Patrick and Jeremy F. Keys, and colleagues

Department of Biomedical Engineering, University of Kentucky, Lexington, KY, USA

Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)

Published in Scientific Reports, September 2022

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#science #biomedicine #immunofluorescence #microgels #bones #blood vessels #blood supply #endothelial #mesenchymal stem cells

[kosheeka]

Why GMP Compliance Is Paramount For High-Quality Mesenchymal Stem Cell?

The past twenty years have witnessed a fascinating unboxing of the mesenchymal stem cells. These microscopic marvels hold the potential to revolutionize how we approach disease. These mesenchymal stem cells are extracted from adult cells, and hence, they rarely receive any ethical backlash. Unlike most stem cells, mesenchymal ones boast remarkable versatility and can morph into diverse cell types, from bone to blood vessels. These cells carry several regenerative and anti-inflammatory prowess, which has propelled them to the forefront of stem cell therapy. The use in therapeutic applications has ignited a surge in demand that outpaces our current production capabilities. 

Given the surge in demand for MSCs for research, providing researchers with high-quality stem cells for reproducible research is necessary. Enter the realm of Good Manufacturing Practices (GMP), our roadmap towards building factories for these cellular powerhouses, ensuring not just quantity but unparalleled quality and safety. Buckle up, science researchers, for we’re about to delve into the intricate dance of scaling up MSC production while upholding the highest standards, paving the way for a future where these microscopic maestros weave their magic on a grand scale.

#Mesenchymal Stem Cells #MSCs #GMP Compliance Is Paramount For High-Quality Mesenchymal Stem Cell #Challenges With Mesenchymal Stem Cells #Cell culture #customized primary cells #primary cells #biotech company #stem cells #exosomes #stem cell research center #regenerative medicine #bioengineering #Kosheeka

[mindfulcells]

Stemirac® is a PMDA approved stem cell therapy for spinal cord injury that is showing promising results in clinical trials. Mesenchymal stem cells (MSCs), like those used in Stemirac®, can help to repair damaged tissue and improve quality of life for patients with spinal cord injury.

#spinal cord injury #stem cell therapy #pmda #mesenchymal stem cell #recovery #treatment for spinal cord injury #regenerative medicine #tissue regeneration #japan #spine #spinehealth #spinalcord #cell therapy #regenerativemedicine #health #stem cells #health education #spinetreatment #healthcare #hope for the future #conditionalapproval #regulatory approval

[poojaj]

Mesenchymal Stem Cells (MSC) Market to Witness Huge Growth by 2030

The mesenchymal stem cells (MSC) market refers to the commercialization and distribution of mesenchymal stem cells for various applications in regenerative medicine and cell therapy. Mesenchymal stem cells are a type of adult stem cell that can differentiate into multiple cell types, such as bone cells, cartilage cells, and fat cells. They have the potential to repair and regenerate damaged tissues and have gained significant attention in the field of regenerative medicine.

For Sample Report Click Here:- https://www.marketinforeports.com/Market-Reports/Request-Sample/511529

The MSC market has been growing rapidly in recent years due to the increasing interest and investment in regenerative medicine and cell therapy. MSCs have shown promise in treating various medical conditions, including orthopedic injuries, cardiovascular diseases, autoimmune disorders, and neurological disorders. They can be obtained from different sources, including bone marrow, adipose tissue, umbilical cord blood, and placenta.

Several factors have contributed to the growth of the MSC market. These include the increasing prevalence of chronic diseases and degenerative disorders, rising geriatric population, advancements in stem cell research and technologies, and favorable regulatory environments for cell-based therapies in some countries.

The market for MSCs includes both research and clinical applications. In the research sector, MSCs are used for studying stem cell biology, developing new therapies, and screening drug candidates. In the clinical sector, MSCs are being tested in various clinical trials and are being used in approved therapies in some countries.

The MSC market is highly competitive, with numerous companies and research institutions involved in the development, manufacturing, and distribution of MSC-based products. Some companies specialize in the isolation and expansion of MSCs from different sources, while others focus on developing specific therapies or treatment protocols. Additionally, there are companies offering storage and banking services for MSCs.

#Mesenchymal Stem Cells (MSC) Market #Mesenchymal Stem Cells (MSC)

[usasportsmedicine]

This stem cell invasive treatment evolved over the past years. Stem cell treatment or Mesenchymal stem cell therapy in South Florida is one of the best alternatives to any surgical option with a fast recovery process. If you are going through any untreatable issues, you can avoid them through stem cell treatment. Let's check some more benefits of receiving stem cell therapies

#Mesenchymal stem cell therapy in South Florida

[asploroopenaccesspublications]

The Mechanism of Propofol on Non-Small Cell Lung Cancer (NSCLC) through Modulating Mesenchymal Transition (EMT) | Abstract

The Mechanism of Propofol on Non-Small Cell Lung Cancer (NSCLC) through Modulating Mesenchymal Transition (EMT) | Abstract

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#Cancer Stem-Like Cells (CSCS) #Mesenchymal Transition #Non-Small Cell Lung Cancer #Propofol #Self-Renewal Capacity

[spacetimewithstuartgary]

New stem cell research to take flight into space

A Mayo Clinic research experiment will be part of a payload that launches into space from NASA's Kennedy Space Center in Cape Canaveral, Florida, on Jan. 29, subject to weather conditions and other factors. The research team from the lab of Dr. Abba Zubair is preparing stem cells for the flight to test how the absence of gravity plays a role in bone loss.

"We've known for some time that astronauts lose bone density on long-duration space flights," says Dr. Zubair, a laboratory medicine and pathology specialist at Mayo Clinic in Florida. "We want to understand how this occurs so we can work on solutions that prevent bone loss not only in astronauts while they're in space, but also in patients here on Earth."

The research project will examine the effect of gravity on a type of stem cells derived from bone marrow known as mesenchymal stem cells, or adult stem cells with growth factors and healing potential. They play a key role in tissue repair and regeneration. Dr. Zubair's team will look at how the stem cells function while in space. Dr. Zubair says the experiment could have implications for future space flights that include taking humans to Mars.

This early research also could affect human clinical trials down the road, perhaps a decade away, according to Dr. Zubair.

"We will use what we learn from this project to advance our research on the road to clinical trials, with the ultimate goal of testing therapeutic agents that can prevent or treat bone loss that comes with osteoporosis, as well as bone loss that occurs in patients who are bedridden for long periods of time," he says.

The research will be conducted during two space flights. The first space flight will evaluate the impact of microgravity on bone-forming stem cells. The second space flight is tentatively planned for the end of the year and will analyze the effect of microgravity on other cell types that participate in bone formation or loss. A compound will be tested that can be used to treat bone loss in space and potentially on Earth.

The first foray into space for Dr. Zubair's research was in 2017, when a payload of several samples of donated stem cells blasted off from Kennedy Space Center in a test to determine if they could hold up in space and be more quickly mass-produced in microgravity for use in stroke treatment. A subsequent research experiment in space found that stem cells grown in weightlessness are safe and feasible for applications to human disease.

Mayo Clinic is collaborating with Bioserve Space Technologies in Boulder, Colorado, which is providing the space flight hardware for the research project.

#science #space #astronomy #physics #news #astrophysics

[lifealteringstemcells]

Stem Cell Therapy: Recent Advancement For Spinal Cord Injuries

A spinal cord injury (SCI) is damage caused to the nerves in the spinal cords that are responsible for sending and receiving signals from the brain. The traumatic lesion in the spinal cord can cause permanent or irreversible sensory and motor deficits. Stem cell therapy has shown massive potential in repairing damaged tissues and promoting recovery of neurological function. Medical research shows the effectiveness of stem cell therapy in treating various neurodegenerative disorders like ischemic stroke, intracerebral hemorrhage, and spinal cord injury.

Causes Of Spinal Cord Injury

The increasing number of cases of spinal cord injuries worldwide has made SCI a global health priority. Sports injuries and road accidents have been recognized as the two common causes of SCI. Although traumatic conditions comprise major triggering factors to induce spinal cord damage, non-traumatic events such as inflammation, spinal disc degeneration, cancer, substantial tissue loss, and infections can also be the reason for injury to the spinal cord and peripheral nervous system,

Symptoms of Spinal Cord Injury

The emergency symptoms of SCI may include loss of control in body movements due to partial or complete loss of sensory function. It is characterized by loss of motor control of the back, arms, legs, and other body parts, leading to weakness and incoordination. Patients experience extreme pain and pressure in the back, neck, and head.

Other prominent symptoms that doctors have identified as effects of spinal cord damage are -

Difficulty in walking and sitting

Difficulty in breathing

Sudden reflexes or spasms

Loss of control over bladder and bowel movements

Lowered sexual sensitivity and infertility

Trouble in balancing

Numbness or tingling sensation in the hands, fingers, and feet

The Science of Stem Cell Therapy For Spinal Cord Injury Treatment

Spinal cord injury is a severe condition that damages the nerve cells and tissues in the spinal cord, resulting in paralysis or nervous function impairment. Current treatment approaches include medication, rehabilitation therapy, physical therapy, or surgery. However, most of them provide temporary relief and poor outcomes in the long run. Stem cell interventions have emerged as a promising treatment for SCI with its exemplary potential to repair and regenerate injured neurons and tissues.

The distinct ability of stem cells to proliferate and form any functional cell type makes them ideal for treating SCI. The cells self-renew into nerve cells to replace and repair the diseased cells. After they are introduced into a patient’s body, they reach the injured site and interact with the surrounding cells to produce neurotrophic growth factors and alter the microenvironment of the affected region in the spinal cord.

A preclinical study has shown that mesenchymal stem cells release growth-promoting factors that accelerate the growth of axons at the injured area and improve myelination (formation of specialized membranes around axons). Most importantly, the therapeutic potential of stem cells prevents further neuronal degeneration in the spinal cord, lowering inflammation and improving motor and sensory function.

Start Your Stem Cell Therapy Journey In Mexico

There is no better place than Mexico for stem cell therapy because this North American country is home to highly qualified and experienced medical professionals with a proven track record of successful cell therapies. Besides, the healthcare system in Mexico is also advanced, and its treatment solutions are most accessible and affordable to people all over the world.

Life Altering Stem Cell Therapy Institute is a trusted name if you are ready to receive stem cell therapy in Mexico. Their state-of-the-art technologies, innovative treatments, and comfortable clinic setup have earned them the tag of stem cell therapy best hospital in Mexico. Their health advocates are just a call away! Connect with them to learn more about stem cell therapy for treating spinal cord injuries and other chronic conditions.

#stem cell treatment #stem cell therapy #stem cell research

[kosheeka]

Impact of Cell Source on Tissue Engineering Applications

In recent years, tissue engineering has shown tremendous promise for a wide range of applications, including regenerative medicine, drug discovery, and toxicology testing. However, the success of tissue engineering relies heavily on finding the best sources of cells to use in the process. The choice of cell source is crucial for the success of tissue engineering and can impact the efficacy and safety of the final product.

Read more:- https://kosheeka.com/impact-of-cell-source-on-tissue-engineering-applications/

#Tissue engineering #types of cells #Embryonic stem cells #Adult stem cells #Induced pluripotent stem cells #Mesenchymal stem cells #stem cells availability #stem cell therapy #stem cell treatment #primary cells #regenrative cells #bio-engieering

[hoursofreading]

The female reproductive system more broadly is also misunderstood. The generally accepted narrative imagines the sperm as the hunter, while the egg is the passive and lucky object of its manly chase. But Clancy rightly gives these “ideas about eggs as princesses in a tower and sperm as rescuing princes” short shrift. The ovaries don’t simply release a chosen follicle (a sac of fluid containing an egg) into the fallopian tube right before ovulation. Instead, they ruthlessly “oversee continual, overlapping waves of competition to select” the best follicle to release. The cervix has crypts where it stores sperm “to use later … to prevent overcrowding at the egg and allow for some selection of preferred sperm.” Gamete fusion is a tango, not a one-way assault. This poor understanding of the female body has consequences. Only recently have scientists discovered that menstrual blood may speed up skin repair because it contains powerful mesenchymal stem cells. Clancy adds that menstrual effluent, which is made up of blood, endometrial tissue, cells, biomarkers, and hormones, also contains important antimicrobials and antioxidant enzymes. And had more scientists been willing, like Clancy, to take a “deep dive into menstrual effluent,” we might have understood sooner that the menstrual cycle can cause a spike in an inflammatory biomarker called C-reactive protein (CRP). Elevated CRP is also used to diagnose people as prediabetic. How many women who thought themselves prediabetic were actually just menstruating? Female hormones don’t skew data. They are data.

#quote

[advancexo]

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#regenerativemedicine #antiaging #exosomes #cosmeticformulations #cosmeticsindustry #skincareproducts

[didanawisgi]

Effects of intentionally treated water on the growth of mesenchymal stem cells: An exploratory study

Highlights

•Intentionally treated water appeared to have some biological effects on the growth, pluripotency and senescence of human primary mesenchymal stem cells (MSCs).

•Primary cells obtained from two donors were designated as Cells #1 and Cells #2. Proliferation averaged across Cells #1 and #2 showed overall increased growth in treated as compared to control water (p = 0.0008).

•For Cells #1 considered separately, no differences in gene expression levels were significant except for p16, which resulted in an effect opposite to the predicted outcome (p = 0.05). For Cells #2, three genes expressed significantly in the predicted directions: NANOG (p = 0.0008), OCT4 (p = 0.005), and P53 (p = 0.05); p16 was significantly opposite to the prediction (p = 0.001).

Abstract

Objective

This study explored if human primary mesenchymal stem cells (MSCs), derived from two donors and cultivated in a medium made with intentionally treated water, would exhibit more growth and pluripotency than MSCs from the same source but grown in untreated (control) water.

Design

To create the treated water, three Buddhist monks directed their attention toward commercially bottled water while holding the intention that the water would enhance the growth of MSCs. Under double-blind conditions, cell culture growth mediums were prepared with the treated and untreated water, which was in turn used to grow the primary MSCs. Primary cells obtained from two donors were designated as Cells #1 and Cells #2. The prediction was that treated water would result in increased cell proliferation, that more cells would enter the cell cycle growth phase, and that there would be increased expression of genes (NANOG, OCT4 and SOX2) associated with improved cell growth and decreased expression of genes (p16, p21, and p53) associated with a decline in cell growth. The improved growth hypothesis was directional, thus one-tailed p-values were used to evaluate the results.

Results

Proliferation averaged across Cells #1 and #2 showed overall increased growth in treated as compared to control water (p = 0.0008). Cells #1 and #2 considered separately had differences in the same direction but only Cells #2 showed a significant difference on day 6 (p = 0.01). For cell cycle, there was a significantly greater percentage of Cells #2 in the S interphase with treated vs. control water (p = 0.04). For the gene expression analysis, when considering the average across the two donor cells, only the NANOG gene expression was in the predicted direction (p = 0.01); by contrast, the p16 gene expression was significantly opposite to the predicted direction (p = 0.005, one-tailed, post-hoc). For Cells #1 considered separately, no differences were significant except for p16, which resulted in an effect opposite to the predicted outcome (p = 0.05). For Cells #2, three genes were significantly in the predicted directions: NANOG (p = 0.0008), OCT4 (p = 0.005), and P53 (p = 0.05); p16 was significantly opposite to the prediction (p = 0.001).

Conclusion

Intentionally treated water appeared to have some biological effects on the growth, pluripotency and senescence of human MSCs. This was especially the case in one of the two donor cells tested, but the effects were not consistently in the predicted direction. As an exploratory study, caution is warranted in interpreting these outcomes, and adjustment for multiple testing would likely reduce some of the weaker effects to nonsignificant. But given the double-blind protocol, as well as several more significant outcomes in the predicted directions, further research is warranted.

#Dean Radin #quantum biology #quantum consciousness #fav #alchemy

[news-archiv24]

Human bone marrow-derived mesenchymal stem cells (hBMSCs) and their derivative enhanced green fluorescent protein (eGFP)-hBMSCs were employed to evaluate an innovative hybrid scaffold composed of granular hydroxylapatite and collagen hemostat (Coll/HA). The cellular morphology/cytoskeleton organization and cell viability were investigated by immunohistochemistry (IHC) and AlamarBlue metabolic assay, respectively. The expression of osteopontin and osteocalcin proteins was analyzed by IHC and ELISA, whereas osteogenic genes were investigated by quantitative PCR (Q-PCR). Cell morphology of eGFP-hBMSCs was indistinguishable from that of parental hBMSCs. The cytoskeleton architecture of hBMSCs grown on the scaffold appeared to be well organized, whereas its integrity remained uninfluenced by the scaffold during the time course. Metabolic activity measured in hBMSCs grown on a biomaterial was increased during the experiments, up to day 21 (p < 0.05). The biomaterial induced the matrix mineralization in hBMSCs. The scaffold favored the expression of osteogenic proteins, such as osteocalcin and osteopontin. In hBMSC cultures, the scaffold induced up-regulation in specific genes that are involved in ossification process (BMP2/3, SPP1, SMAD3, and SP7), whereas they showed an up-regulation of MMP9 and MMP10, which play a central role during the skeletal development. hBMSCs were induced to chondrogenic differentiation through up-regulation of COL2A1 gene. Our experiments suggest that ...

#stem cells #therapie #tissue engineering

[campbellmedicalclinic]

How The Regenerative Medicine in Houston Redefining Healthcare Solutions

In the heart of Texas, a healthcare revolution is underway, redefining traditional medical solutions and offering renewed hope to patients seeking innovative treatments. At the forefront of this transformative movement lies Regenerative Medicine in Houston, where pioneering approaches are changing the landscape of healthcare as we know it.

Regenerative medicine represents a paradigm shift, moving away from merely treating symptoms to fostering the body's natural ability to heal and regenerate. This progressive field encompasses a range of cutting-edge therapies aimed at repairing, replacing, or regenerating cells, tissues, or organs to restore normal function.

One notable institution leading this charge is the Campbell Health Center, a beacon of excellence in regenerative medicine. With a commitment to patient-centric care and a multidisciplinary approach, the center is dedicated to harnessing the power of regenerative therapies to address a myriad of health conditions.

What sets Campbell Health Center apart is its integration of advanced technologies and evidence-based practices to deliver personalized treatment plans tailored to each patient's unique needs. Whether it's utilizing stem cell therapy to promote tissue regeneration or deploying platelet-rich plasma (PRP) injections to accelerate healing, the center's holistic approach emphasizes both efficacy and safety.

In recent years, regenerative medicine has garnered attention for its potential to revolutionize the treatment of orthopedic injuries, chronic pain, and degenerative conditions. Through innovative techniques such as mesenchymal stem cell therapy and regenerative orthopedics, patients are experiencing improved outcomes and enhanced quality of life, often without the need for invasive surgeries or prolonged medication regimens.

Moreover, regenerative medicine holds promise in addressing a wide range of medical challenges beyond orthopedics. From autoimmune disorders to neurological conditions, researchers are exploring the therapeutic potential of regenerative therapies in diverse fields, offering hope to patients with previously untreatable conditions.

The impact of regenerative medicine extends far beyond individual patients, influencing the broader healthcare landscape in Houston and beyond. By shifting focus from reactive to proactive care, regenerative therapies have the potential to reduce healthcare costs, minimize the burden of chronic disease, and improve overall population health.

At Campbell Health Center, this forward-thinking approach is evident in its collaborative efforts with leading researchers, healthcare professionals, and industry partners. Through ongoing research initiatives and clinical trials, the center remains at the forefront of innovation, driving progress in regenerative medicine and shaping the future of healthcare delivery.

As awareness and acceptance of regenerative medicine continue to grow, so too does the demand for accessible and comprehensive care. Campbell Health Center is poised to meet this demand, providing patients with unparalleled expertise, state-of-the-art facilities, and compassionate support every step of the way.

In conclusion, the emergence of regenerative medicine in Houston represents a transformative milestone in healthcare, offering new possibilities for healing, recovery, and wellness. With institutions like Campbell Health Center leading the charge, patients can look forward to a future where innovative therapies pave the way for healthier, more vibrant lives. As the journey towards regenerative healthcare unfolds, the promise of hope and healing shines brighter than ever before.

#Campbell Health Center #Regenerative Medicine Houston #Healthcare #Treatment #Health #Fitness

[gloriouscreationtyrant]

[kosheeka]

Despite the specific application or molecule to be analyzed, certain essential aspects should be considered while constructing an in vitro assay.

#stemcells #research #kosheeka #regenerativemedicine #primarycells #cellculture #cellbiology #healthcare #healthresearch

#Stem Cells #hematopoietic stem cells #mesenchymal stem cells #cancer stem cells