#ecorie

[proxentauri]

the second half to the collab i did with the fantastic @sophios-draws!! this time i did the sketch/lineart and they did the colouring; check out the other version here!

the characters are my designs of a humanized version of the EcoRI (right) and FokI restriction enzymes, based on soph's original designs! i'll post refs for them at some point, probably (explaining how their character designs correspond to the enzymes' method of action)...check out soph's blog for other cool bio humanizations!!

#art tag #ecori #foki #collab #sophios #biology #enzymes #science side of tumblr #i think

[chiknluvr]

How does one...?

(feel free to fill in the blank before answering, or not, it's up to you)

…two, three, four?

jokes aside, i can tell you how NOT to run EcoRI protocol

don’t. fucking. accidentally cook it at 65C for an hour and a half instead of 37.5C

#long day equals silly mistakes #oh well #it’s tomorrow’s problem now

[indolentinsect]

to piggy back off my last post, i took the lamdaphage genome sequence (retrieved from NCBI website) and put it into NEBcutter3.0 and did a custom digest with EcoRI and selected the gel view to compare my experimental results against. my digest was run on the far left, so i can see that it went pretty well :)

#college #biology #science #university #student #microbiology #studyblr #laboratory #lab assistant #bacteria #virus #bacteriophage #molecular biology

[wakewithgiggli]

#copyright #piracy

[finitepeace]

december reads: xiewei x jiang xuening <from story of kunning palace>

The Fantastic Mr. Zhang by ecorie | G, 2k words, post-canon |

Prime Minister Xie Wei is not above sending Zhang Zhe to prison for his "crimes against humanity". His father-in-law Minister Jiang, near retirement but still in full possession of his faculties and impeccable memory, thinks what goes around comes around. Yan Lin thinks he really need to teach his son not to start family drama.

#cdrama #cdrama fic rec #story of kunning palace #xie wei #jiang xuening

[ambientalmercantil]

#E-MOBILIDADE #ENERGIASOLAR #GERAÇÃODISTRIBUÍDA #GESTÃODEENERGIAS

[regioonlineofficial]

Dit is de belangrijkste conclusie uit het onderzoek dat de Rekenkamer Zeist heeft laten doen naar de stand van zaken wat betreft de energietransitie in Zeist. De ambities zijn er zeker en de wil om er te komen ook, maar de gemeente zal het tempo moeten opschroeven om de doelen te kunnen halen. Het hoofddoel is om in 2050 volledig energieneutraal te zijn en voor één derde in 2030 We hebben steeds vaker te maken met gevolgen van de klimaatverandering. Energietransitie is van groot belang om de impact van klimaatverandering te verminderen en een toekomstbestendige wereld voor volgende generaties te waarborgen. Daar zullen alle gemeenten aan moeten bijdragen. Het onderwerp is actueler dan ooit en ook bij de gemeente Zeist heeft het onderwerp de volle aandacht. Via twee paden uit de Routekaart Nieuwe Energie wil de gemeente de doelen bereiken: energiebesparing aan de ene kant (Warme Wijken genoemd in de routekaart) en duurzame energieopwekking (Duurzame Bronnen) aan de andere kant. Alle inwoners en ondernemers in Zeist krijgen met energietransitie te maken. Het is in ieders belang dat er vaart gemaakt wordt. Dit heeft de rekenkamer doen besluiten om te onderzoeken hoe het er nu in Zeist voor staat met de uitvoering van de energietransitie, of de doelen haalbaar zijn en waar eventueel aanpassingen of versnellingen wenselijk zijn. De rekenkamer heeft twee inwoners uit Zeist gevraagd om als ‘wisselspelers’ voor dit onderzoek mee te denken en mee te praten. Voor het eerst hebben we een jongere bij het onderzoek betrokken. Het is immers hun toekomst waar het over gaat. Onderzoek Rekenkamer Uit het onderzoek, dat op verzoek van de Rekenkamer uitgevoerd is door Ecorys, komt naar voren dat er in Zeist al veel wordt gedaan aan (het bevorderen van) energiebesparing maar dat het huidige tempo niet hoog genoeg is om de doelen te halen. Hier moet een tandje bij. De gestelde doelen voor duurzame opwekking lijken zelfs helemaal niet haalbaar volgens de onderzoekers. Dat komt voor een deel omdat Zeist niet genoeg ruimte heeft voor wind- en zonne-energie. Daarbij speelt ook het landelijk probleem van de netcongestie een rol, die nieuwe aansluitingen belemmert. Met andere woorden: niet alles ligt in deze in de handen van de gemeente, maar het vraagt wel om een reflectie op de gestelde doelen, leidend tot een nieuwe strategie, en om afstemming en samenwerking met de buurgemeenten via de Regionale Energie Strategie. De onderzoekers constateren wel dat Zeist heeft een sterke uitgangspositie heeft voor succesvolle transitie, door een heldere beleidsvisie en de wil om er te komen, maar ook dankzij initiatiefrijke inwoners en actieve maatschappelijke organisaties die hierbij behulpzaam zijn. 

[surveycircle]

Tweeted

Recherche de participants pour une étude ! Sujet : "Transformation & Innovation RH – Les nouvelles méthodes de recrutement" https://t.co/0UYRwK3ZL0 via @SurveyCircle #recrutement #MarketingRh #MarqueEmployeur #InboundRecruitment #ecoris #enquête #surveycircle https://t.co/b4a8oVTNnf

— Daily Research @SurveyCircle (@daily_research) May 6, 2023

#IFTTT #Twitter

[barkerweber13]

Reactive Sulfur Species: A brand new Redox Player throughout Cardio Pathophysiology.

Results of check details this study show in which investigation associated with way of life supernatants just before cellular hair transplant offers helpful biomarkers showing the niche associated with cells utilized for transplantation.Fourier enhance infrared (FTIR) spectroscopy as well as a selection of FTIR gun rings have already been employed to examine the structure and also family member flexibilities conferred by simply different flanking sequences on the EcoRI binding website. This method granted people to check distinctive peaks along with subtle changes ill the particular spectra regarding n(AAAGAATTCTTT)(2), deb(TTCGAATTCGAA)(Only two), along with deb(CGCGAATTCGCG)(Only two) and therefore determine community modifications in starting integrating, starting piling, spine conformation, glycosidic relationship turn, and sweets puckering inside the Analyzed sequences. The alterations in flanking sequences cause variations the particular sugars puckers, glycosidic connect turn, along with anchor conformations. Various amounts of neighborhood freedom tend to be observed inside series in complete agreement together with prior neurological action assays. The outcomes provide promoting proof to the presence of the splay inside the H(Four)-C(Nine) bottom pair of the actual EcoRI holding site along with a probable bank account involving versatility with the H(Some) Bosom web site that have been recommended inside the materials. In summary, we now have demonstrated that FTIR is really a powerful method pertaining to studying the effect of flanking series on DNA structure and adaptability, for it provides details about the local composition from the nucleic chemical p as well as the overall comparable flexibilities conferred through distinct flanking patterns.History: Contributor heart malfunction (DHD) precluding purchasing pertaining to hair transplant is situated around 25% of brain-dead (BD) contributors. The molecular components of DHD remain unclear. Many of us researched the possibility part regarding myocardial interleukin (Illinois)-6 signaling through the JAK2-STAT3 path, resulted in the actual generation regarding nitric oxide supplements (Absolutely no) and also diminished heart myocyte contractility. METHODS: Hearts have been procured utilizing normal approach with School of Iowa (UW) option through 15 bestower using a quit ventricular (LV) ejection portion regarding <35% (DHD). 15 minds along with regular operate (NF) following BD dished up because handles. LV IL-6 ended up being quantitated by enzyme-linked immunoassay (ELISA) as well as JAK2-STAT3 signaling was evaluated by simply appearance regarding phosphorylated STAT3. Inducible NO synthase (iNOS) and caspase-3 had been measured by simply exercise assays. RESULTS: Myocardial IL-6 appearance had been 8-fold greater inside the DHD group compared to NF settings. Phosphorylated STAT3 term had been 5-fold greater in DHD compared to NF, implying improved JAK2-STAT3 signaling. LV exercise associated with iNOS was 2.5-fold increased in DHD compared to NF. LV term in the pro-apoptotic gene Bnip3 as well as caspase-3 activity have been 3-fold higher inside the DHD team than in the actual NF party. CONCLUSIONS: Myocardial IL-6 term is quite a bit larger from the setting involving DHD in comparison with kisses obtained along with typical operate.

#MS4078 #LY3214996 #PF-00835231 #Lifirafenib #TLR2-IN-C29 #UNC0638 #DC661 #DRB18 #SH-4-54 #Elesclomol #UAMC-3203 #Laduviglusib #TBK1/IKKε-IN-1 #Mizagliflozin #BIX 02189 #Milciclib #Diroximel #Rimiducid #PFK15 #BAY 87-2243 #XMD8-92 #MRTX-1257 #OTSSP167 #PF-06882961 #Z-YVAD-FMK #Dyngo-4a #MT-802 #THZ531 #ALW II-41-27 #Pyrrolidinedi

[petrosolgas]

A Ecori Energia Solar está recrutando consultor, motorista e especialista no setor energético para compor o seu quadro de funcionários

A Ecori Energia Solar está recrutando consultor, motorista e especialista no setor energético para compor o seu quadro de funcionários

Atuando há mais de dez anos no mercado energético brasileiro, a Ecori Energia Solar busca expandir cada dia mais. Levando a energia solar de qualidade, ao mesmo tempo em que se preocupa com o meio ambiente e em fornecer oportunidades para os brasileiros que atuam no setor. Dessa forma, nessa sexta-feira, (05/08), há algumas vagas de emprego sendo ofertadas para aqueles que desejam ingressar em…

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[switchbush4]

Adenylosuccinate synthase restrains cancer of the colon mobile viability, motility along with epithelial‑mesenchymal move along with tumorigenesis within unclothed rodents using the PI3K/AKT path

KOK reduced the elevated mRNA phrase associated with pro-inflammatory cytokines (IL-1 experiment with, IL-6, TNF-alpha), chemokines (IL-8, MCP-1), and iNOS within the ovaries, along with greater the lower mRNA appearance involving development factors (EGF, TGF-b) simply by DHEA shot. Post-administration of KOK in addition improved the particular DHEA-induced PCOS-like signs or symptoms, generally much like these apparent from pre-administration regarding KOK. KOK might successfully avoid along with improve DHEA-induced PCOS via anti-inflammatory motion, showing #Link# its precautionary and restorative prospect of suppressing Polycystic ovarian syndrome.hspA encodes a little temperature surprise proteins (sHSP) throughout Xanthomonas campestris pv. campestris, the particular causative adviser of dark-colored decay throughout cruciferous crops. In this research, two-dimensional carbamide peroxide gel electrophoresis, marketer activity assays, and N . hybridization results said that HspA expression was brought on by simply high temperature shock but not simply by other strains, though low-level appearance has been evident simply by reverse transcription-polymerase chain reaction (RT-PCR) under normal way of life conditions. An hspA mutant showed diminished tolerance to high temperature, mainly in the existence of MgSO(Four), however absolutely no change in pathogenicity. Outcomes of size-exclusion chromatography established that purified HspA(his), containing six C-terminal histidine residues, created a couple of different measurement instructional classes of oligomeric complexes-410 and also 820 kDa. In contrast, HspA(ter), the actual unmodified proteins converted from the initial hspA gene, created exactly the 820-kDa complicated. These types of benefits suggest that the particular C-terminus associated with HspA is essential pertaining to oligomerization. The two HspA820(their) and also HspA410(his or her #Link# ) had the ability to somewhat protect luciferase versus heat-induced gathering or amassing. As opposed to other documented sHSPs which typically get denaturing meats throughout refoldable declares until finally refolded by adenosine triphosphate-dependent chaperone systems, HspA(his / her) on your own ended up being competent at reactivating heat-inactivated EcoRI. As a result, Xanthomonas campestris pv. campestris HspA features prospective program as being a protecting broker during the storage space associated with meats.Many experts have proven in which hang-up associated with de novo sphingolipid activity #Link# increases insulin shots level of sensitivity. For even more quest for the particular device required, many of us utilized two designs: heterozygous serine palmitoyltransferase (SPT) subunit Only two (Sptlc2) gene knockout these animals along with sphingomyelin synthase A couple of (Sms2) gene knockout mice. SPT is paramount compound in sphingolipid biosynthesis, and Sptlc2 is among it's subunits. Homozygous Sptlc2-deficient rats tend to be embryonic lethal. Even so, heterozygous Sptlc2-deficient mice which were workable along with with out significant developing disorders demonstrated lowered ceramide and sphingomyelin amounts in the cellular plasma filters, and also heightened level of sensitivity in order to blood insulin. In addition, these types of mutant rats have been protected against high-fat diet-induced weight problems along with blood insulin weight. Text may be the previous compound regarding sphingomyelin biosynthesis, along with SMS2 is among it's isoforms. Sms2 insufficiency greater mobile or portable tissue layer ceramide yet decreased SM quantities. Sms2 deficit furthermore elevated the hormone insulin awareness along with ameliorated high-fat diet-induced unhealthy weight.

#Cisplatin #Ibrutinib #Adenylosuccinate synthase

[indolentinsect]

gel electrophoresis of lamdaohage DNA digested with EcoRI and HindIII :)

EcoRI digested DNA is in the first and third columns, HindIII digested DNA is in the second and fourth column.

this was done as part of the cell & molecular bio class i’m taking

#college #biology #science #university #student #microbiology #studyblr #laboratory #lab assistant #bacteria #virus #dna #enzymes

[dailyjobse]

International Development job at Ecorys International Development

International Development job at Ecorys International Development

Vacancy title: International Development [ Type: FULL TIME , Industry: Manufacturing , Category: Management ] Jobs at: Ecorys International Development Deadline of this Job: 09 May 2022 Duty Station: Within Zambia , Lusaka , South – Central Africa Summary Date Posted: Thursday, May 05, 2022 , Base Salary: Not Disclosed JOB DETAILS: We are currently have an opportunity for a Regional Business…

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[drwcn]

*Navigation*

~ESTABLISHED FICS~

{Begotten} — completed, on AO3.

{Alone Stands the Quiet} — completed, Begotten’s sequel, on AO3

{The Good Arion} — completed, on AO3. 

{The Shadow Beneath the Light} — completed, #tsbl, on AO3

~~~

{Before the Sun Falls} —  navigation page or AO3 :)

{Discordance} — navigation page or AO3. :) 

~FICLET SERIES~

#cql ficlet

{Deep Waters} — completed, dark!lxc, master list

{A Willing Hostage} — completed, nmj bridenaps jyl, master list

{Remnant} — completed, dark!lan brothers au #2, master list

{Midnight Sun} — completed, fem!wwx + wangxian kid, master list

{The Red Regent} — completed, evil!wen qing au, master list

{Without Envy} — completed, concubine/spy!wwx & prince!lwj, master list

~~~

{Courtship & Cultivation} — p&p x the untamed, #ppcql

{Carbon in the Steel} —dark!lan brothers au #1, master list

~TAGS~

cql reference - information about cdrama world building (fashion, language, culture etc). 

~ Corie :)

#about corie #corie talks #ecories #ecorie #pinned post #i have been told tumblr is impossible to navigate so here you go #you can always come back to this #this will be pinned to the front of my blog!!

[ambientalmercantil]

#ENERGIASOLAR #GESTÃODEENERGIAS

[wangxianficrecs]

❤️Begotten by ecorie

❤️Begotten 

by ecorie

G, 37k, wangxian, background xiyao

Summary:  “He’s mine.” He echoed what had once been teasingly said in jest, and added, “This is my son.”

Against all odds and without a choice, Lan Zhan brings A-Yuan back to Cloud Recesses. Xichen keeps his brother’s secrets, and shields the child when Lan Zhan could not.

My comments:  Warning: This is gonna put you through the wringer. Just so you know. All the anguish and helplessness and abiding love and... it's delicious in how much it'll hurt. I say this only 5 chapters in, and it's unfinished, so who knows. Well, we all know, which is to say, we have to wait for 13 years before wwx returns, and it's gonna be hard sailing for Lan Wangji until then. POV shifts between lwj and Lan Xichen (who only wants to help his brother, because as he points out to Lan Qiren, he's only sect leader until tides change or something catastrophic happens, but he will ALWAYS be brother to lwj) and Lan Sizhui, once he's older.

Lan Zhan returns with Wen Yuan and hands him over to his brother to protect before he heads off to his discipline whipping. Of course, that doesn't go so well, and it's months before he regains consciousness, even. But he only has a short time with A-Yuan before he's in seclusion, and meanwhile Xichen tries to keep all his balls in the air and not collapse under the strain.

Story covers the 13-year interval, and now my heart hurts and I've been crying, but it's all very tasty, so....

Also, I'm super curious about Lan Sizhui's unusual cultivation and what he'll be able to do with resentful energy!

Excerpt:  And that pain, it must’ve bled through the hollowness of his gaze, because Xichen let out a sigh, morose and resigned. The older Lan took a step towards the younger, arms open and ready with acceptance.

“Give him to me.”

Lan Zhan blinked, a little caught off guard. His grip on A-Yuan tightened, and he could not stop himself from pulling away. The ‘no’ was on the tip of his tongue before his brother’s pleading expression gave him pause.

“As he is your son, then I am his uncle. Brother, surely you would not think me so low as being capable of allowing any harm to come to my own nephew.”

Xichen was calm, determined. Thank the gods, because Lan Zhan needed that now more than anything. Slowly, he relented, passing the feverish child over to Xichen. “A-Yuan is ill. He needs medicine urgently.”

A-Yuan?

Given the severity of the boy's condition, Xichen would not normally trouble with the legalities right then, were he not certain that the disciplinary measures waiting for his brother would incapacitate him without mercy. If the boy was to be inaugurated into the clan, certain procedures and due processes were necessary, and certain facts - like name and parentage - would need to be charted for the records.

It hurts to think it, but this would be the last of Lan Zhan's conscious moments for a long time to come. Xichen had born some vain hope that the lashes could somehow be avoided, or at the very least lessened, but he realized now that he must face the inevitable, bravely too, for a small innocent life- and the last piece of Wangji's fractured heart he suspected - had been entrusted to him. The punishment was not only inescapable, but had become necessary to provide for an opportunity - a distraction - so that Xichen may quietly and inconspicuously induct the child into the ranks.

"A-Yuan; is that his name?"

Name? Ah yes, name. Lan Zhan nodded. "Yes. A-Yuan…Lan Yuan."

family drama, family feels, brotherly feels, angst, FEELS, lan wangji is a good dad, lan xichen is a good uncle, 13 years, 33 lashes, hurt lan wangji, hurt/comfort, discipline whip, emotional hurt/comfort, adoption, abusive Lan Sect, grief/mourning, loneliness, isolation, character study, protective lan wangji, protective lan xichen, parent-child relationship, lan sizhui, growing up, special core, dual core, demonic cultivation, favorite

(You may wish to REBLOG as a signal boost for the author if you like -- or think others might like -- this story.)

#Wangxian Fic Rec #Wangxian #brotherly feels #family feels #angst #lan wangji is a good dad #13 years #33 lashes #hurt lan wangji #hurt/comfort #emotional hurt comfort #abusive lan sect #grief/mourning #favorite #special core #parent-child relationship #Begotten #ecorie #medium fic 15k-49k #General