Fun Facts about Fireflies and Fluorescence under the cut :)
FUN ANIMAL FACT: Fireflies glow due to a chemical reaction between the molecule Luciferin and the enzyme Luciferase (and quite a few other molecules including ATP and oxygen). While fireflies use their bioluminescence for mating reasons, the reaction can be used in a test named the -ATP fluorescence assay- to detect bacteria in various bodily samples and monitor levels of photosynthesis in crop fields (although it was originally developed to potentially detect living cells on Mars)
In 1955, Shimomura joined the lab of Yoshimasa Hirata at Nagoya University, where he was tasked with extracting the organic compound luciferin from ostracods and determining its precise molecular structure. Today, scientists know that in many bioluminescent organisms, an enzyme named luciferase catalyzes a chemical reaction between luciferin and oxygen, which produces light. But back then, “we didn’t fully understand how it happened,” Shimomura says. “It was a mystery.” After 10 months of labor in the lab, Shimomura became the first person to crystallize luciferin, an essential step in studying its structure.
In the 1960s, he continued his research at Princeton University, where he also started to investigate the luminous jellyfish Aequorea victoria. Shimomura and his colleagues collected numerous A. victoria specimens and strained them like cider apples to obtain a small amount of pure glowing “squeezate.” Within the shining liquid they discovered a protein they named aequorin, which releases blue light when it reacts with calcium, even in the absence of oxygen. Another protein in the jellyfish, green fluorescent protein (GFP), sometimes absorbs that blue light, and releases green light in response.
By 1978, after collecting nearly a million jellyfish, Shimomura had thoroughly elucidated the structure of aequorin and the nature of A. victoria’s unique light-generating reactions. Both aequorin and GFP—as well as the gene coding for the latter—became indispensable tools in biology and medicine. Scientists could now tag and observe the intricate dances of previously invisible genes and proteins in living cells. In 2008, along with Martin Chalfie of Columbia University and Roger Tsien of the University of California, San Diego, Shimomura received the Nobel Prize in Chemistry for his work on GFP.
More cameraless exposures of fireflies. A few months ago, we were fortunate to spend time together instead of remotely. We capped it off by returning to one of our early collaborative projects, started in the mid-90's. A firefly was gently collected and taken into a darkroom. 'Blinky' was placed into a cigar box that had a piece of 4x5 sheet film inside. It did its thing by pulsing bioluminescent light directly onto the film emulsion. You can even see its legs. Basically a photogram; no lens was used. When we felt it did enough, the film was removed. Afterwards, we thanked Blinky and let that bug go free... 2022 #fireflies #lenslessphotography #experimentalphotography #photograms #cameralessphotography #largeformatphotography #naturephotography #lightningbugs #firefly #analogphotography #abstractphotography #bioluminescent #abstractfilmphotography #abstractart #alternativephotography #contemporaryphotography #luciferase #collaborativeart #naturalworld #experimentalfilm #glowworm #outdoorphotography #luciernaga #bioluminescence #alternativeprocesses #collaborativephotography #bioluminiscencia #insectphotography #bichitosdeluz #glowinthedark https://www.instagram.com/p/CiQgwiNpP07/?igshid=NGJjMDIxMWI=
Being a firefly squid and all that, Marie’s tentacles contain luciferase (the glowy enzyme that causes stuff to… well, glow). The levels of luciferase fluctuate based on hormonal and emotional factors, so she only glows sometimes.
Norwegian butter squares, and I’ll be honest I can’t think of much else just because I don’t normally eat anything with coffee. Butter squares are great though because they’re light and fluffy, not super sweet. I have the recipe saved somewhere…
16. 3 drinks you consume the most
Coffee, water and teas of various flavours!
27. 3 things you wish you did more often
I wish I was more spontaneous and stopped overthinking every action, I wish I could show myself more authentically and I wish I did more yoga!
Here’s what’s happening: The group of chemicals involved to make plankton glow are broadly termed luciferins and the light is produced by a series of oxidation reactions set off by a catalyst called luciferase. The bioluminescence in plankton is very high in several forms of Plankton and is a form of cold light or luminescence.
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Video credit: unknown (please DM me if you know/are the creator)